目的 构建OSKM诱导的过表达c-Jun肝癌细胞（C3A-c-Jun）重编程细胞系，探讨外源性c-Jun对肝癌细胞重编程的影响。 方法 通过C3A细胞稳定过表达c-Jun后进行OSKM诱导重编程，通过碱性磷酸酶染色，Real-time PCR，免疫印迹法，免疫荧光等技术对细胞进行鉴定，建立C3A-c-Jun诱导肿瘤干细胞系C3A-c-Jun-iCSCs。 结果 C3A-c-Jun-iCSCs克隆呈圆顶状，边缘圆钝，克隆内细胞小且排列紧密，多层分布，碱性磷酸酶染色阳性，mRNA和蛋白水平均可表达多潜能性标记物OCT4、SOX2;C3A-c-Jun-iCSCs组外源性和内源性Sox2的表达量均明显高于C3A-iCSCs对照组;在重编程过程中c-Jun持续表达。结论 OSKM诱导C3A、C3A-c-Jun肝癌细胞重编程，分别建立C3A-iCSCs和C3A-c-Jun-iCSCs细胞系，发现外源性c-Jun通过上调Sox2基因的表达，启动下游一系列反应，对肝癌细胞重编程过程起促进作用。

Objective To establish an OSKM induced C3A-c-Jun of liver cancer reprogrammed cell line, and to explore the effects of exogenous c-Jun on liver cancer cell reprogramming. Methods OSKM was utilized to induce C3A-c-Jun to reprogram. Alkaline phosphatase staining, Real-time fluorescent quantitative polymerase chain reaction(Real-time PCR), Western blotting, and immunofluorescence technology were used to identify cells, and establish the C3A-c-Jun-iCSCs. Results C3A-c-Jun-iCSCs cells formed dome-shaped clones, in which cells were small, closely packed and multi-layered. Alkaline phosphatase staining was positive. They expressed pluripotent markers Oct4, Sox2, Nanog at the mRNA level and express OCT4, and SOX2 at the protein level. C3A-c-Jun-iCSCs had obviously higher gene expression of Sox2 compared with C3A-iCSCs, whatever in exogenous expression or endogenous expression. C-Jun continually expressed in the process of reprogramming. Conclusion OSKM induces C3A/C3A-c-Jun of liver cancer reprogramming and built the C3A-i-CSCs/C3A-c-J-un-iCSCs. Exogenous c-Jun has a promoting effect on liver cancer cell reprogramming process by upregulating Sox2, which then can activate downloaded genes.