线粒体转录终止因子蛋白家族的表达以及在MPTP / MPP+ 诱导下的表达变化

张林冰 韩艳艳 仇石 许珊 左伋 刘雯*

doi:10.16098/j.issn.0529-1356.2017.01.004

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解剖学报 ›› 2017, Vol. 48 ›› Issue (1) : 19-24. DOI: 10.16098/j.issn.0529-1356.2017.01.004

神经生物学

线粒体转录终止因子蛋白家族的表达以及在MPTP / MPP⁺ 诱导下的表达变化

张林冰韩艳艳仇石许珊左伋刘雯*

作者信息 +

Expression and alteration induced by MPTP / MPP⁺of mitochondrial transcription termination factors family

ZHANG Lin-bing HAN Yan-yan QIU Shi XU Shan ZUO Ji LIU Wen*

Author information +

文章历史 +

摘要

目的探讨线粒体转录终止因子（MTERFs）蛋白家族在组织和细胞中的表达以及在神经毒素MPTP/MPP⁺诱导下的表达变化。方法取正常C57BL小黑鼠的脑、肝、肾组织，以及人神经母细胞瘤细胞SH-SY5Y、人心肌细胞HCM、人肝细胞L-02，检测MTERFs的蛋白表达情况；通过MPTP/MPP⁺构建帕金森病（PD）动物/细胞模型，检测MTERFs的蛋白表达变化情况。结果 MTERFs在小鼠脑中和SH-SY5Y细胞中蛋白表达水平较高；MPTP/MPP⁺ 诱导的PD模型中，MTERF3蛋白表达水平明显降低。结论在小鼠脑和SH-SY5Y细胞中，MTERFs表达较高；MPTP/MPP⁺诱导下MTERF3蛋白表达明显降低，影响了线粒体转录水平，这可能参与了帕金森病发生的过程。

Abstract

Objective To investigate the expression of mitochondrial transcription termination factors (MTERFs) in various tissues and cells, and the changes of MTERFs induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)/1-methyl-4-pheny lpyridine(MPP⁺ ). Methods Western blotting was used to measure the expression of the MTERFs in brain, liver, kidney of C57BL mice and in human SH-SY5Y, HCM, L-02 cells. MPTP/MPP⁺ was injected to imitate PD animal/cell models. The changes of MTERFs protein were detected through western blotting. Results The expression of MTERFs was higher in mice brain and SH-SY5Y cells. In PD mouse model induced by MPTP, MTERF3 reduced significantly. Conclusion The expression of MTERFs was higher in mouse brain and SH-SY5Y cells. After induced by MPTP/MPP⁺ , MTERF3 decreased obviously, which increased transcription level and might involve in a process of Parkinson's disease.

导出引用

张林冰韩艳艳仇石许珊左伋刘雯. 线粒体转录终止因子蛋白家族的表达以及在MPTP / MPP⁺ 诱导下的表达变化[J]. 解剖学报. 2017, 48(1): 19-24 https://doi.org/10.16098/j.issn.0529-1356.2017.01.004

ZHANG Lin-bing HAN Yan-yan QIU Shi XU Shan ZUO Ji LIU Wen. Expression and alteration induced by MPTP / MPP⁺of mitochondrial transcription termination factors family[J]. Acta Anatomica Sinica. 2017, 48(1): 19-24 https://doi.org/10.16098/j.issn.0529-1356.2017.01.004

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