目的 探讨白藜芦醇预处理对大鼠皮质神经元氧糖剥夺/再复氧损伤后神经元突起生长的影响。方法 体外皮质神经元氧糖剥夺150min，复氧培养24 h。实验分为正常组、对照组和5μmol/L白藜芦醇预处理组。免疫荧光法鉴定神经元，细胞计数试剂盒8（CCK-8）法测定细胞活力，TUNEL法检测细胞凋亡，免疫荧光法和Western blotting检测微管相关蛋白2（MAP-2）和生长相关蛋白43（GAP-43）的表达,并计数神经元突起的长度和数目。结果 培养细胞均高表达神经元特异性标记物MAP-2。白藜芦醇组细胞活力较对照组明显增加（0.551±0.009 比 0.436±0.013, P<0.01），而凋亡则明显降低（18.3%±1.3% 比35.3%±1.9%, P<0.01），上调MAP-2(0.790±0.102 比 0.462±0.063, P<0.01)和GAP-43(0.768±0.084 比 0.424±0.065,P<0.01)蛋白的表达，增加神经元突起的长度(89.510±6.939 比 61.538±9.14,P<0.01)和数目(6.347±1.002 比 3.040±0.608,P<0.01）。结论 白藜芦醇预处理能减轻氧糖剥夺/再复氧对神经元的损伤，促进神经元突起的生长。

Objective To investigate the effects of resveratrol pretreatment on neurite growth of rat primary cortical neurons after oxygen-glucose deprivation/reperfusion (OGD/R) injury in vitro. Methods Primary cortical neurons were cultured under oxygen and glucose deprivation for 150 minutes and reoxygenation for 24 hours. The study had the normal, control and 5μmol /L resveratrol pretreatment groups. Neurons were identified with immunofluorescence. Cell viability was detected with cell counting kit-8(CCK-8) assay. Cell apoptosis was detected with TUNEL assay. Immunofluorescence and Western blotting measured the expressions of microtubule-associated protein 2（MAP-2）and growth associated protein 43（GAP-43）, and the length and number of neurites were counted.
Results Cells had high expression of neuronal specific marker MAP-2. Compared with the control group, resveratrol treatment significantly enhanced the neurons viability (0.551±0.009 vs 0.436±0.013,P<0.01), decreased the numbers of apoptosis (18.3%±1.3% vs 35.3%±1.9%,P<0.01), upregulated the expressions of MAP-2 (0.790±0.102 vs 0.462±0.063,P<0.01) and GAP-43 (0.768±0.084 vs 0.424±0.065,P<0.01) proteins, increased the length (89.510±6.939 vs 61.538±9.14,P<0.01) and numbers (6.347±1.002 vs 3.040±0.608,P<0.01) of neurites. Conclusion Resveratrol pretreatment can reduce injury and promote neurite growth of cultured neurons after OGD/R.