新生大鼠心telocytes的形态学和蛋白标记

甘黎 常玉巧 张三林 牛艳菲 余霞 郭志坤*

doi:10.16098/j.issn.0529-1356.2016.05.009

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解剖学报 ›› 2016, Vol. 47 ›› Issue (5) : 633-637. DOI: 10.16098/j.issn.0529-1356.2016.05.009

细胞和分子生物学

新生大鼠心telocytes的形态学和蛋白标记

甘黎¹常玉巧¹张三林² 牛艳菲¹余霞¹ 郭志坤^1*

作者信息 +

Morphology and protein labeling of telocytes in cardiac neonatal rats

GAN Li¹ CHANG Yu-qiao¹ZHANG San-lin² NIU Yan-fei¹ YU Xia¹GUO Zhi-kun^1*

Author information +

文章历史 +

摘要

目的探讨新生大鼠心telocytes（TCs）的形态学特征并验证其是否表达神经细胞、神经胶质细胞的特异性标记。方法酶消化法培养新生大鼠原代细胞，免疫细胞化学、扫描电子显微镜鉴定TCs，免疫荧光技术检测TCs的神经细胞和神经胶质细胞的蛋白表达。新生大鼠大脑组织免疫组织化学染色作为阳性对照。结果免疫组织化学检测显示波形蛋白（vimentin）表达阳性，免疫荧光检测vimentin和CD34共表达，但TCs内神经元特异性烯醇化酶（NSE）、GFAP、OX42、2’3’-环腺苷酸-3’-磷酸二酯酶（CNpase）表达阴性。扫描电子显微镜下TCs形态清晰典型，特征形态明显。结论大鼠心 TCs虽然具有与神经细胞类似的形态，但其并不具有神经细胞的特性。

Abstract

Objective To study the morphological characteristics of telocytes（TCs）in neonatal rats and to verify whether TCs express the special markers of neural cells and glial cells. Methods Cardiac TCs were isolated and cultured from neonatal rats with collagenase digestion method. Immunocytochemistry, immunofluorescence and scanning electron microscopy were used to identify TCs, and to detect whether TCs had characteristics of neural and glial cells. Neonatal rat brain tissue was used as a positive control. Results Cardiac TCs were in a good state and of high purify. The immunocytology staining showed that cultured cells positively expressed vimentin and co-expressed vimentin and CD34 rather than neuron-specific enolase(NSE), glia fiber acidic protein(GFAP), OX42 and 2’3’-cyclic nucleotide 3’-phosphodiesterase (CNpase). The morphology of TCs was clear and distinguishable under a scanning electron microscope. Conclusion Cardiac TCs do not have the characteristics of neuronal and glial cells though they have some similar morphology.

导出引用

甘黎常玉巧张三林牛艳菲余霞郭志坤. 新生大鼠心telocytes的形态学和蛋白标记[J]. 解剖学报. 2016, 47(5): 633-637 https://doi.org/10.16098/j.issn.0529-1356.2016.05.009

GAN Li CHANG Yu-qiao ZHANG San-lin NIU Yan-fei YU Xia GUO Zhi-kun. Morphology and protein labeling of telocytes in cardiac neonatal rats[J]. Acta Anatomica Sinica. 2016, 47(5): 633-637 https://doi.org/10.16098/j.issn.0529-1356.2016.05.009

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