大鼠白色和棕色脂肪来源的间充质干细胞成脂分化特性的比较

牛艳菲 赵国安 常玉巧 甘黎 殷国田 郭志坤*

doi:10.16098/j.issn.0529-1356.2016.05.005

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解剖学报 ›› 2016, Vol. 47 ›› Issue (5) : 607-613. DOI: 10.16098/j.issn.0529-1356.2016.05.005

细胞和分子生物学

大鼠白色和棕色脂肪来源的间充质干细胞成脂分化特性的比较

牛艳菲¹ 赵国安²常玉巧¹ 甘黎¹殷国田² 郭志坤^1*

作者信息 +

Differences of adipogentic differention characteristics between the mesenchymal stem cells from white adipose tissue and brown adipose tissue in rats

NIU Yan-fei¹ ZHAO Guo-an² CHANG Yu-qiao¹ GAN Li¹ YIN Guo-tian² GUO Zhi-kun ^1*

Author information +

文章历史 +

摘要

目的探讨白色脂肪组织（WAT）和棕色脂肪组织（BAT）来源的间充质干细胞成脂分化特性的差异。方法雄性SD大鼠15只，3只用于细胞分离培养，12只用于细胞移植。体外分离培养WAT和BAT两种来源的脂肪干细胞，用油红O染色检测两种干细胞的成脂分化率，用免疫荧光技术检测成脂诱导、分化后的细胞是棕色脂肪细胞还是白色脂肪细胞。4’6-二脒基-2-苯基吲哚（DAPI）标记两种来源的干细胞后移植至腹股沟区，分别在第1、2、3周取材，免疫荧光技术检测植入细胞的分化趋向。结果 WAT来源的干细胞增殖速度明显快于BAT来源的干细胞，前者成脂分化率为0.205±0.069，后者为0.165±0.053，两种干细胞的成脂诱导率差异无统计学意义（P>0.05）。两种干细胞分别植入体内后，在第1、2、3周发现，两种干细胞均表达棕色脂肪特异性蛋白解耦联蛋白1（UCP1）。结论 WAT和BAT来源的干细胞在体外及体内诱导成脂后均分化为棕色脂肪细胞。

Abstract

Objective To study differences of adipogenic differentiation characteristics between the mesenchymal stem cells from white adipose tissue (WAT) and brown adipose tissue (BAT). Methods Fifteen male SD rats were used in this study, 3 for cell separation and 12 for cell transplantation. Adipose mesenchymal stem cells from WAT and BAT were separated and cultured in vitro. Oil red O staining was used to test the adipogenic differentiation rate of each kind of stem cells. Immunofluorescence techniques was used to test the adipogenic differentiation rate of each kind of stem cells, and to detect whether the cells were white fat cells or brown fat cells after adipogenic induction and differentiation. Both stem cells after 4’6-diamidino-2-phenylindole(DAPI) staining were transplanted to the groin area. Sample tissues were obtained in 1, 2 and 3 weeks. Immunofluorescence technique was used to detect the differentiation trend of the implanted cells. Results Proliferation rate of stem cells from WAT was significantly faster than that of BAT. Adipogenic differentiation rate of the former was 0.205±0.069, while the latter was 0.165±0.053. Adipogenic rates of the two kinds of stem cells had no statistical significance (P>0.05). After adipogenic cells were implanted in the body, brown adipose specific proteins uncoupling protein 1(UCP1) were expressed from two kinds of stem cells in 1, 2 and 3 weeks’ tissues. Conclusion Both stem cells of WAT and BAT cultured in vitro and in vivo were differentiated into brown fat cells after adipogentic induction.

导出引用

牛艳菲赵国安常玉巧甘黎殷国田郭志坤. 大鼠白色和棕色脂肪来源的间充质干细胞成脂分化特性的比较[J]. 解剖学报. 2016, 47(5): 607-613 https://doi.org/10.16098/j.issn.0529-1356.2016.05.005

NIU Yan-fei ZHAO Guo-an CHANG Yu-qiao GAN Li YIN Guo-tian GUO Zhi-kun. Differences of adipogentic differention characteristics between the mesenchymal stem cells from white adipose tissue and brown adipose tissue in rats[J]. Acta Anatomica Sinica. 2016, 47(5): 607-613 https://doi.org/10.16098/j.issn.0529-1356.2016.05.005

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