目的 制备去细胞人羊膜基质（AHAM）并分析其移植至小鼠体内后的免疫反应，评价去细胞人羊膜基质作为细胞移植支架的免疫原性。 方法 利用胰蛋白酶-EDTA消化人羊膜（HAM)，去除人羊膜上皮细胞，制备新鲜去细胞人羊膜基质；新鲜去细胞人羊膜基质置于含硫酸软骨素及甘油的冷冻保护液中，-80℃保存；使用前经PBS复水处理后即冷冻去细胞人羊膜基质；免疫细胞化学分析去细胞人羊膜基质中人类白细胞抗原的表达情况；将冷冻的去细胞人羊膜基质移植至BALB /c小鼠肝脏内4周后，分析脾脏组织中CD4⁺ 、CD8⁺ T淋巴细胞比例的变化。 结果 新鲜及冷冻保存后的去细胞人羊膜基质均不表达人类白细胞抗原；冷冻的去细胞人羊膜基质移植至小鼠体内后，脾脏组织中CD4⁺ 、CD8⁺ T淋巴细胞无明显变化。 结论 去细胞人羊膜基质的免疫原性较低，移植后不会引起由T细胞介导的特异性排斥反应，可以作为免疫安全性的生物支架用于疾病的细胞移植治疗。

Objective To prepare of the acellular human amniotic membrane matrix (AHAM) and to assess the immunogenic property of AHAM as a scaffold for cell transplantation. Methods To prepare the AHAM, the HAM was peeled from the placenta, cut into pieces and incubated in trypsin with EDTA for decellularization. For generating cryopreserved AHAM, the fresh AHAM pieces were placed in dishes with a 1∶1 mixture of glycerol and chondroitin sulfate in MEM-NEAA and stored at -80℃. Before using, the cryopreserved AHAMs were rehydrated with sterile PBS. The expression of human leucocyte antigen in HAM and AHAM was determined by immunofluorescence. The ratio of CD4⁺ and CD8⁺ T cells in mouse spleen after AHAM transplantation for four weeks was assessed by immunofluorescence and flow cytometry. Results Immunofluorescence analysis confirmed that the fresh and cryopreserved AHAM were negative for human leucocyte antigen antibody. Immunofluorescence and flow cytometry analysis confirmed that the ratio of CD4⁺ and CD8⁺ T cells in mouse spleen after AHAM transplantation was not changed. Conclusion The immunogenic property of AHAM is relatively low and does not cause the T cells mediated immunological rejection after transplantation in the mouse model. These results support the potential of AHAM as a cell delivery platform to treat and manage disease.