目的 探讨RNAi靶向沉默对人卵巢癌OVCAR3细胞CD105和Ki67基因的表达。 方法 采用脂质体介导的基因法将不同浓度的CD105-siRNA、 Ki67-siRNA转染至体外培养的人卵巢癌OVCAR3细胞中，检测干扰前后人卵巢癌细胞中CD105、Ki67基因表达的变化以确定沉默效果。构建CD105-siRNA、Ki67-siRNA及各自的阴性对照序列并高效地转染人卵巢癌OVCAR3细胞，采用Western blotting和Real-time PCR从蛋白和基因水平检测CD105和Ki67的表达变化。 结果 转染CD105-siRNA或Ki67-siRNA终浓度为50nmol/L和100nmol/L的人卵巢癌细胞中CD105mRNA或Ki67mRNA的相对表达水平及CD105和Ki67的表达均明显低于空白对照组和阴性对照组（NC1），差异具有统计学意义（P＜0.001）。 结论 CD105-siRNA和Ki67-siRNA能特异性抑制人卵巢癌OVCAR3细胞中的CD105和Ki67基因表达，下调mRNA和蛋白的表达水平。

Objective To detect changes of the CD105 and Ki67 gene expression and to determine the effect of gene silence on OVCARs cells transfected these gene sequences by liposome lipofectamine TM2000 respectively. Methods OVCAR3 were transfected with siRNA of CD105-siRNA group, Ki67-siRNA group and negative control groups in vitro respectively. The expressions of CD105mRNA, Ki67mRNA and their proteins were detected with Real-time PCR and Western blotting. Results The intensities of relative expressions for both CD105 mRNA and Ki 67mRNA were significantly lower in both siRNA-CD105 transfected and siRNA-Ki67-transfected OVCARs than those in control groups(P<0.001). Conclusion CD105-siRNA and Ki67-siRNA can specifically inhibit CD105, Ki67 gene expression levels in ovarian cancer OVCAR3 cells and lowered their mRNA and protein expression.