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肾性高血压大鼠左心室整合素 β1和黏着斑激酶的表达
Expression of integrin β1 and focal adhesion kinase of the left ventricule in renal hypertensive rats
目的 探讨肾性高血压大鼠左心室壁重构过程中整合素β1（integrin β1）和黏着斑激酶（FAK）的表达变化。 方法 采用双侧肾动脉狭窄法制作动物模型，40只SD大鼠随机分为假手术组（sham）、术后2周组、术后5周组和术后7周组，血压及左心室重量指数评价造模成功后，取左心室组织，采用HE染色观察心肌细胞的形态变化，免疫组织化学和Western blotting检测各组左心室肌组织integrin β1和FAK的表达。 结果 HE染色结果显示，实验组大鼠左心室组织的心肌纤维走向紊乱，心肌细胞间隙变大；integrin β1和FAK蛋白分别表达于心肌细胞膜和心肌细胞质近膜区，呈线样或细颗粒状分布；integrin β1的表达强度在术后随时间呈逐渐上升趋势，至5周组趋于平稳；FAK在2周组的表达强度相对于假手术组明显下降（P<0.01），实验组随术后时间增加表达量增强；Western blotting结果显示，integrin β1和FAK在各手术组的表达均明显高于假手术组（P<0.01），5周组较2周组下降明显（P<0.01），7周组和5周组差异不明显（P>0.05）。 结论 Integrin β1和FAK在心室肌的表达变化可能是高血压心肌肥厚的重要原因。
Objective To explore the variation of integrin β1 and focal adhesion kinase（FAK）during left ventricular wall re-modeling in the rats with renal hypertension.
Methods The animal model was prepared by bilateral renal artery stenosis, and renal artery was freed in the control group. Forty rats were randomly divided into sham operation group, and two-week group, five-week group and seven-week group after operation. The model quality of hypertensive rat was assessed by a noninvasive blood pressure analysis tester and the index of left ventricular weight (LVW/BW). The morphological changes of the left ventricle was observed under a light microscope after HE staining. The immunohistochemistry and Western blotting methods were used to detect integrin β1 and FAK distribution in the ventricle muscle tissues. Results HE staining showed that the myocardial fibers were disordered and the space between the myocardial cells increased. The positive signals of both of integrin β1 and FAK immunohistochemistry were distributed in myocardium. The intensities of the immunohistochemical positive staining profiles for both integrin β1 and FAK increased with time and reached equilibrium for 5weeks treatment by image analysis. Average absorbance(AA) value of FAK expression at the 2nd week was declined significantly than the control group and increased with time in operation groups. Western blotting showed that the expression level of integrin β1 and KAK in every operation group were all higher than sham group (P<0.01). Five-week group was declined significantly compared with 2-week group (P<0.01) and no significance difference between 7-week and 5-week group（P＞0.05）.FAK had a significantly lower expression level in all operation groups than control group, 5-week group was declined significantly compared with 2-week group (P<0.01) and no significance difference between 7-week and 5-week group（P＞0.05）. Conclusion The abnormal expression of integrin β1 and FAK in the left ventricular may be an indication of myocardial hypertrophy.
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