欢迎访问《解剖学报》官方网站！今天是
English
贵州某白酒对大鼠睾丸间质细胞内类固醇激素急性调节蛋白表达及血清睾酮水平的影响
Effect of Guizhou liquor on rat Leydig cells in the expression of steroidogenic acute regulatory protein and serum testosterone level
目的 探讨摄入贵州某54°白酒后，不同时程及不同剂量对大鼠睾丸间质细胞内类固醇激素急性调节蛋白（StAR）表达及血清睾酮水平的变化，以期为科学饮酒提供基础资料。 方法 正常成年雄性SD大鼠69只，随机分为正常对照组（n=15）及实验组（n=54）。实验组分为低剂量组0.8ml/（kg·d）、中剂量组1.6ml/（kg·d）及高剂量组2.4ml/（kg·d），实验组大鼠每日灌胃2次。分别于第4周末、第8周末、第12周末采血清，取睾丸组织。 采用免疫组织化学SABC法、图像分析、Western blotting检测睾丸组织StAR的表达，采用化学发光法检测大鼠血清睾酮水平。 结果 各实验组的血清睾酮水平与正常对照组比较未见明显异常，差异无统计学意义（P>0.05）。StAR免疫组织化学阳性产物存在于睾丸间质细胞胞质内；与正常对照组比较，4周、8周、12周各剂量组StAR免疫染色及平均吸光度均有所增强（P<0.05）； Western blotting检测，4周、8周、12周各剂量组StAR蛋白表达水平增强（P<0.05）。 结论 在本实验设定的剂量和时程内用该白酒灌胃后，大鼠睾丸间质细胞内StAR表达水平有所增强，血清睾酮水平并无明显变化。
Objective To investigate effects of a Guizhou 54 degrees liquor on rat leydig cells in the expression of steroidogenic acute regulatory protein (StAR) and the change of the serum testosterone level at the different time and with different doses. Methods Sixty normal adult male SD rats were randomly divided into a normal control group(n=15) and an experimental group(n=54). Each experimental group was further subdivided into low dose group 0.8 ml/（kg·d）, middle dose group 1.6 ml/（kg·d）, high dose group 2.4 ml/（kg·d）. The rats of the experimental groups were given by gavage twice a day(11∶00 AM an 5∶00 PM)with liquor. At the end of the 4th, 8th and 12th week, the serum was collected and testicular tissue was taken. The expression of StAR was detected by immunohistochemical SABC method, image analysis and Western blotting, and level of the serum testosterone was detected by Chemiluminescence method.
Results StAR immunohistochemical positive products existed within the cytoplasm of Leydig cells; Compared with the normal control group, the average absorbance of StAR staining in the experimental groups at the 4th, 8th, and 12th week increased(P<0.05). Western blotting showed that the expression level of StAR protein in the experimental groups at the 4th, 8th and 12th week increased(P<0.05). Compared with normal control group, serum testosterone level of each experimental group did not see apparently unusual,and there was no statistically significant difference(P>0.05). Conclusion In this experiment a set of doses and schedules after gavage with the liquor enhance the StAR expression level in the rat Leydig cells enhancement.Serum testosterone level has no obvious change.
［1］Zhao XJ, Sun YCh, Qu YE, et al. Effects of Heshouwuyin on the expression of P450 side-chain cleavage enzyme and steroidogenic acute regulatory protein in testis tissue of the exercised-induced fatigue rat［J］. Acta Anatomica Sinica, 2012, 43(4): 524-529. (in Chinese)
赵秀军, 孙一翀, 曲银娥, 等. 何首乌饮对运动疲劳大鼠睾丸组织 P450胆固醇侧链裂解酶和类固醇激素急性调节蛋白的影响［J］. 解剖学报, 2012, 43(4): 524-529.
［2］Lin ChY, Liu HY, Tao XQ, et al. Effects of annexin 5 on the expressions of StAR, P450scc and 3β -HSD in the testis of SD rats［J］. Journal of Medical Postgraduate, 2011, 24(4): 340-345. (in Chinese)
林钗英, 柳海燕, 陶晓倩, 等. 膜联蛋白5对雄性SD大鼠睾酮合成相关蛋白和酶表达的影响［J］. 医学研究生学报, 2011, 24(4): 340-345.
［3］Clark B, Stocco DM. Steroidogenic acute regulatory protein the StAR still shines brightly［J］. Mol Cell Endocrino, 1997, 134(1): 1-8.
［4］Wu Q, Zhu W, Wang W, et al. Effect of yeast species on the terpenoids profile of Chinese light-style liquor［J］. Food Chem, 2015, 168: 390-395.
［5］Zhang ZhY, Fan WL, Xu Y, et al. Comparative analysis of free amino acid content and composition in different aroma type Chinese liquors ［J］. Science and Technology of Food Industry, 2014, 35(17): 280-284, 288.
［6］Zhao L,Wang PP, Huang CC, et al. Application of Vis/NIR spectroscopy for Chinese liquor discrimination［J］. Food Anal Methods, 2014, 7(6): 1337-1344.
［7］Xiao Z, Yu D, Niu Y, et al. Characterization of aroma compounds of Chinese famous liquors by gas chromatography-mass spectrometry and flash GC electronic-nose［J］. J Chromatogr B Analyt Technol Biomed Life Sci, 2014, 945946: 92-100.
［8］Liu ChY, Lu Y, Yang LL, et al. Study on the damage of germ cells in the male rats caused by alcohol［J］. Chinese Journal of Family Planning, 2004, 4: 215-217. (in Chinese)
刘长云, 卢映, 杨利丽, 等. 酒精致雄性大鼠生殖细胞损伤的实验研究［J］. 中国计划生育学杂种志, 2004, 4: 215-217.
［9］Wang YF, Su JM. Effects of alcohol abuse on male reproductive system［J］. Journal of Medical Forum, 2010, 31(11): 79-80.(in Chinese)
王跃峰, 苏锦明. 酗酒对男性生殖系统的影响［J］. 医药论坛杂志, 2010, 31(11): 79-80.
［10］Jensen TK, Swan S, J?rgensen N,et al. Alcohol and male reproductive health: a cross-sectional study of 8344 healthy men from Europe and the USA［J］. Hum Reprod, 2014, 29(8): 1801-1809.
［11］Li ZhY, Wang XL. Mechanisms of benefits to heart of moderate intake of alcohol［J］. Adv Cardiovasc Dis, 2007（4）：647-650. (in Chinese)
李制英，王晓丽. 适量饮酒有益心脏的机制［J］.心血管病学进展，2007（4）：647-650.
［12］Gao YM, Li H. Histology and Embryology［M］. Beijing: People’s Medical Publishing House, 2005: 281-282.(in Chinese)
高英茂, 李和. 组织学与胚胎学［M］. 北京：人民卫生出版社, 2005: 281-282.
［13］Barbara J, Elizabeth A, StAR protein stability in Y1 and kin-8 mouse adrenocortical［J］. Biology (Basel), 2015, 4（1）: 200-215.
［14］Bose HS, Lingappa VR, Miller WL. The steroidogenic acute regulatory protein, StAR, works only at the outerm itochondrialm embranre［J］. Endocr Res, 2002, 28(4): 295-308.
［15］Hum DM, Miller WL. Transcriptional regulation of human genes for steroidogenic enzymes［J］. Clin Chem, 1993, 39(2): 333-340.
［16］Miao J, Chan KW, Chen GG, et al. Blocking BRE expression in Leydig cell inhibits steroidogenes is by downregulating 3 betahydroxy steroid dehydrogenase［J］. J Endocrinol, 2005, 185(3): 507-517.
［17］Stocco DM. StAR protein and the regulation of steroid hormone biosynthesis［J］. Annu Rev Physiol, 2001, 63(1): 193-213.
［18］Wang XZh, Pan HM, Sun Y, et al. HCG acutely regulating testosterone’s biosynthesisby increasing the expressing of StAR protein［J］. Acta Veterinaria et Zootechnica Sinica, 2005,36(8): 789-793. (in Chinese)
王鲜忠, 潘红梅, 孙燕，等. HCG通过StAR蛋白快速调节睾酮的合成［J］. 畜牧兽医学报, 2005, 36(8): 789-793.
［19］Payne AH, Youngbod GI. Regulation of expression of steroidogenic enzymes in Leydig cell［J］. Biol Reprod, 1995, 52(2): 517-225.
贵州省教育厅“125”重大科技专项
/
| 〈 |
|
〉 |
