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分泌性腹泻对小鼠钙激活Cl-通道基因表达的影响
Effects of secretory diarrhea on calcium activated chloride channel gene expression in the mouse
目的 探讨肠道组织钙激活Cl-通道（CLCAs）基因表达与分泌性腹泻发生的关系。 方法 选取昆明小鼠24只，雌雄各半，随机分为3组：对照组、实验1h和8h组，每组8只。对照组小鼠经腹腔注射0.2ml生理盐水，实验组小鼠经腹腔注射脂多糖（LPS，6mg/kg）分别作用1h和8h，于注射后观察小鼠活动特征及肠道组织形态，以判定分泌性腹泻模型的建立，利用实时光定量PCR法检测各段肠道组织CLCAs基因的表达。 结果 LPS成功诱导小鼠发生了分泌性腹泻；小鼠十二指肠、空肠、回肠和结肠中均有CLCAs基因的转录；注射LPS后，十二指肠、空肠、回肠和结肠的CLCA1、CLCA2、CLCA3、CLCA4、CLCA6基因转录水平均发生上调，与1h组相比，8h组各基因转录水平显著上调。注射LPS后，十二指肠未检测到CLCA5的表达，但空肠、回肠CLCA5的表达均上调。 结论 LPS引起的分泌性腹泻与各肠段CLCAs基因的差异表达有关。
Objective To investigate effects of secretory diarrhea on calcium activated chloride channel（CLCAs）gene expression in the mouse intestinal tissue. Methods Twenty-four Kunming mice (12 males and 12 females) were randomly divided into three groups (n=8 in each group): control, experimental 1 hour and 8 hours groups. The control group were given a normal saline intraperitoneal injection of 0.2 ml, the experimental group were treated an by intraperitoneal injection of lipopolysaccharide (LPS，6 mg/kg) for 1hour or 8hours respectively. After injection for 1 hour or 8 hour, the mouse mental state and intestinal morphology were observed to evaluating secretory diarrhea model establishment. Real-time PCR was used to detect CLCAs gene expression in the intestine. Results The results showed that, LPS induced secretory diarrhea in mice, CLCAs genes were expressed in several intestine segments. After LPS injection, the transcriptional levels of CLCA1, CLCA2, CLCA3, CLCA4, CLCA6 gene in duodenum, jejunum, ileum and colon were up-regulated. Compared with 1 hour group, the gene transcription level significantly up-regulated in 8 hours group. After LPS injection, CLCA5 expression was not detected in duodenum, but expression of CLCA5 was significantly up-regulated in jejunum and ileum. Conclusion The different CLCAs gene expression in intestinal segments is related to secretory diarrhea caused by LPS.
［1］Cottreau J, Tucker A, Crutchley R, et al. Crofelemer for the treatment of secretory diarrhea［J］. Expert Rev Gastroenterol Hepatol, 2012, 6(1):17-23.
［2］Fuller CM, Benos DJ. Ca2+ -activated Cl-channels: a newly emerging anion transport family［J］. News Physiol Sci, 2000, 15(4):165-171.
［3］Hartzell C, Putzier I, Arreola J. Calcium-activated chloride channels［J］. Annu Rev Physiol, 2005, 67: 719-758.
［4］Lorrot M, Vasseur M. How do the rotavirus NSP4 and bacterial enterotoxins lead differently to diarrhea［J］? Virol J, 2007, 4:31.
［5］Wang ChF, Cong YL. Immune protective mechanism of rotavirus infection［J］. China Journal of Preventive Veterinary Medicine, 2005, 27(5): 438-442. (in Chinese)
王春凤, 从彦龙. 轮状病毒感染的免疫保护机制［J］.中国预防兽医学报, 2005, 27(5): 438-442.
［6］Meyerholz DK, Stoltz DA, Pezzulo AA, et al. Pathology of gastrointestinal organs in a porcine model of cystic fibrosis［J］. Am J Pathol, 2010, 176(3):1377-1389.
［7］Jiang L, Zhou MX, Xia PP, et al. All the molecular pathogenic mechanism of enterotoxigenic Escherichia coli infection［J］. China Journal of Veterinary Medicine, 2014, 34(9): 1551-1560. (in Chinese)
蒋磊, 周明旭, 夏芃芃, 等. 产肠毒素大肠杆菌感染的分子致病机制［J］.中国兽医学报, 2014, 34(9): 1551-1560.
［8］Wang YY, Han YQ, Zha GM, et al. Differential expression of CFTR gene in the mouse intestinal tissues［J］. Acta Laboratorium Animals Scientia Sinica, 2014, 22(5): 53-59. (in Chinese)
王月影, 韩莹倩, 查光明, 等. CFTR基因在小鼠肠道组织中的差异表达［J］. 中国实验动物学报, 2014, 22(5): 53-59.
［9］Yang ChY, Han YN, Wang ZX, et al. Effects of 5-HT on stress diarrhea in weaned piglets［J］. Acta Anatomica Sinica, 2015,46(1): 94-100. (in Chinese)
杨晨玉, 韩亚楠, 王子旭, 等. 5-羟色胺对断奶仔鼠应激腹泻的影响［J］.解剖学报, 2015, 46(1):94-100.
［10］Li JQ, Liu X, Xiong XY, et al. Study of rotavirus infection in adult mice［J］. Acta Laboratorium Animals Scientia Sinica, 2004, 12(4):239-243. (in Chinese)
李嘉琦, 刘晓, 熊新宇, 等. 轮状病毒感染成年小鼠的研究［J］.中国实验动物学报, 2004, 12(4):239-243.
［11］Thiagarajah JR, Ko EA, Tradtrantip L, et al. Discovery and development of antisecretory drugs for treating diarrheal diseases［J］. Clin Gastroenterol Hepatol, 2014,12(2):204-209. ［12］Thiagarajah JR, Verkman A. CFTR inhibitors for treating diarrheal disease［J］. Clin Pharmacol Ther, 2012, 92(3):287-290.
［13］Yin L, Vijaygopal P, MacGregor GG, et al. Glucose stimulates calcium-activated chloride secretion in small intestinal cells［J］. Am J Physiol Cell Physiol, 2014, 306(7):C687-696. ［14］Hempson SJ, Matkowskyj K, Bansal A, et al. Rotavirus infection of murine small intestine causes colonic secretion via age restricted galanin-1 receptor expression［J］. Gastroenterology, 2010, 138(7):2410-2417.
［15］Yang N, Lei Z, Li X, et al. Chloroquine stimulates Cl-secretion by Ca2+ activated Clchannels in rat ileum［J］. PLoS One, 2014, 9(1):e87627.
［16］Kunzelmann K, Mall M. Electrolyte transport in the colon: Mechanisms and implications for disease［J］. Physiol Rev, 2002, 82(1):245-289.
［17］Lou T, Yang J, Xu L, et al. Emodin augments ClCa channel in colonic smooth muscle cells［J］. Journal of Mathematical Medicine, 2007, 20(2):143-146. (in Chinese)
娄婷, 杨静, 徐龙, 等. 大黄素增强结肠近端平滑肌细胞钙离子依赖氯离子通道的表达［J］. 数理医药学杂志, 2007, 20(2):143-146.
［18］Puntheeranurak S, Schreiber R, Spitzner M, et al. Control of ion transport in mouse proximal and distal colon by prolactin［J］. Cell Physiol Biochem, 2007,19(14):77-88.
农业部转基因重大专项;郑州市科技攻关计划
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