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突触孔蛋白和细胞色素C在棕榈酸诱导足细胞损伤中的表达及意义
Expression and significance of synaptopodin and cytochrome C in podocytes injuries induced by palmitate acid
目的 探讨细胞骨架蛋白突触孔蛋白（synaptopodin)和细胞色素C（Cyt C）在棕榈酸所致足细胞损伤中的表达及意义。 方法 体外培养小鼠肾足细胞，分为正常对照组和棕榈酸组。各组细胞分别培养 24h、72h和120h。应用MTT方法检测棕榈酸干预后足细胞活性的变化。应用免疫荧光及免疫印迹法检测棕榈酸干预后足细胞中synaptopodin和Cyt C蛋白的表达变化。 结果 与正常对照组相比，棕榈酸组足细胞活性明显下降，且呈时间依赖性。棕榈酸可上调足细胞中Cyt C蛋白的表达，并下调synaptopodin的表达。 结论 棕榈酸可诱导足细胞中Cyt C蛋白释放以及细胞骨架蛋白synaptopodin异常表达，可能参与了足细胞损伤。
Objective To investigate the expression and significance of synaptopodin and cytochrome C(Cyt C) in podocyte injuries induced by palmitate acid. Methods Podocytes were cultured and randomly assigned into two groups, normal group and palimitate acid group.The two groups were cultured for 24hours, 72hours and 120hours. Cell viability of podocytes induced by palmitate acid was examined by MTT. The expressions of synaptopodin and Cyt C of podocytes were examined by immunofluorescence and western blotting. Results Compared with the normal group, cell viability of podocytes was decreased in the palimitate acid group in a time dependence manner. The immunofluorescence and Western blotting results showed that expression of Cyt C was enchanced in podocytes induced by palmitate acid. The expression of synaptopodin was reduced in podocytes induced by palmitate acid. Conclusion Palmitate acid induces that Cyt C is released and synaptopodin is abnormally expressed in podocytes, which may result in podocyte injuries eventually.
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