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坐骨神经分支选择性损伤模型L4~6背根神经节水平不同时间点P2X3mRNA表达变化
P2X3 mRNA expression in L4-6 dorsal root ganglion at different times after the spared nerve injury
目的 观察坐骨神经分支选择性损伤（SNI）动物制作型模后不同时间点L4~6背根神经节（DRG）水平P2X3mRNA的表达情况，探讨外周P2X3mRNA在神经病理性痛模型不同阶段中的作用。方法 54只健康雄性SD大鼠完全随机分为空白对照（control）组、假手术（sham surgery）组和手术（surgery）组。通过结扎腓总神经及切断胫神经，保留腓肠神经的方法建立SNI模型。动态观察造模前、造模后1d、3d、7d和14d双侧足跖机械痛阈；Real-time PCR法检测患侧造模后3d、7d和14d L4~6 DRG水平P2X3mRNA表达情况。结果 模型制作后各时间点，surgery组大鼠患侧足跖痛阈明显降低（P<0.05），sham surgery组大鼠与control组比较差异无显著性（P>0.05）；各组大鼠健侧足跖痛阈于模型制作后各时间点均无显著变化（P>0.05）。模型制作后3d、7d，surgery组大鼠L4、L5、L6 DRG水平P2X3mRNA表达均有不同程度的提高（P<0.05）；而模型制作后14d，surgery组大鼠L5、L6 DRG水平P2X3mRNA表达明显减少（P<0.05），L4 DRG水平P2X3mRNA表达仍显著多于sham surgery组（P<0.05）。模型制作后各时间点、各DRG水平，sham surgery组和surgery组大鼠P2X3mRNA表达差异均无显著性（P>0.05）。结论 外周P2X3mRNA参与SNI模型疼痛的产生和维持，且其在不同阶段发挥的作用不同。
Objective To observe the mRNA expression of P2X3 in L4-6 dorsal root ganglion (DRG) at different times after spared nerve injury (SNI), and to discuss the effect of periphery P2X3 in neuropathic pain. Methods Fifty-four healthy male SD rats were randomly divided into control group, sham surgery group, and surgery group. The spared nerve injury model was established by ligating the common peroneal and the tibial nerves and then cutting off the nerves but keeping the sural nerve intact. Bilateral paw withdrawal thresholds were dynamic observed before injury (base), at day 1, 3, 7 and day 14 after injury. P2X3 mRNA expression in L4-6 DRG at day 3, 7, and day 14 after injury was measured by fluorescence quantitative PCR method. Results Compared with control group, ispilateral paw withdrawal thresholds of surgery group were significantly decreased at day 1, 3, 7, and day 14 after injury (P<0.05), while that of sham surgery had no statistical significance (P>0.05). There was no difference of contralateral pain threshold among control group, surgery group, and sham surgery group (P>0.05). P2X3 mRNA expressions of L4, L5, and L6 DRG of surgery group were remarkable increased at day 3, 7 after injury (P<0.05), while that of L5, L6 DRG were decreased at 14d after injury (P<0.05), and that of L4 DRG were more than that of sham surgery at day 14 after injury (P<0.05). In addition, there was no difference of P2X3 mRNA expression in L4, L5, and L6 DRG at every time after injury between sham surgery group and surgery group. Conclusion Periphery P2X3 mRNA expression is involved in the initiation and maintenance of pain induced by SNI, which may play a different effect at different phases.
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