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磷酸化信号转导和转录激活因子3(Y705)在子宫腺肌症中的表达及其临床意义
Expression of phosphorylated-signal transduction and activator of transcription 3 (Y705) in adenomyosis and their clinical significances
目的 通过检测磷酸化信号转导和转录激活因子3（p-STAT3）（Y705）和血管内皮生长因子（VEGF）在正常子宫内膜与子宫腺肌症在位内膜中的定位与表达，探讨p-STAT3 (Y705)和VEGF在子宫腺肌症中发生发展中的作用。 方法 免疫组织化学SP法检测14例正常子宫内膜和14例子宫腺肌症在位内膜中p-STAT3 (Y705) 和VEGF的定位和表达情况；Real-time PCR检测正常子宫内膜与子宫腺肌症在位内膜组织中STAT3 mRNA 表达情况；Western blotting 检测正常子宫内膜与子宫腺肌症在位内膜组织中p-STAT3 (Y705)、STAT3和VEGF蛋白的表达水平。 结果 p-STAT3 (Y705)主要在正常子宫内膜和子宫腺肌症在位内膜腺上皮细胞核中表达，子宫腺肌症在位内膜蛋白表达高于正常子宫内膜（P＜0.05），正常子宫内膜和子宫腺肌症在位内膜相应的增殖期和分泌期p-STAT3 (Y705)的表达无显著性差异（P＞0.05）。正常子宫内膜和子宫腺肌症在位内膜中，STAT3在mRNA和蛋白表达水平差异均无显著性（P＞0.05）。VEGF主要表达在腺上皮细胞中，子宫腺肌症在位内膜蛋白表达高于正常子宫内膜（P＜0.05），正常子宫内膜和子宫腺肌症在位内膜相应的增殖期和分泌期VEGF的表达差异无显著性（P＞0.05）。
结论 p-STAT3 (Y705) 促进血管生成对子宫腺肌症的发生发展有一定的作用。
Objective To investigate the role of phosphorylated-signal transduction and activator of transcription 3（p-STAT3） (Y705) and vascular endothelial growth factor (VEGF) in the development of adenomyosis by detecting the expression of p-STAT3 (Y705) and VEGF in normal endometrium and eutopic endometrium of adenomyosis patients. Methods The localization and expression of p-STAT3 (Y705) and VEGF in 14 cases of normal endometrium and 14 cases of eutopic endometrium of adenomyosis patients were detected by immunohistochemical SP method.Real-time PCR was performed to understand the expression of signal transduction and activator of transcription 3（STAT3）mRNA in normal endometrium and eutopic endometrium of adenomyosis patients. The protein levels of p-STAT3(Y705) , STAT3 and VEGF in normal endometrium and eutopic endometrium of adenomyosis patients were detected by Western blotting. Results In the normal endometrium and eutopic endometrium of adenomyosis, p-STAT3 (Y705) was mainly located in the nucleus of the glandular epithelium. p-STAT3 (Y705) had higher expression in adenomyosis groups than that in control (P＜0.05). There was no significant difference in the expression of p-STAT3 (Y705) in the proliferative phase and secretory phase of each group (P＞0.05). In the normal endometrium and eutopic endometrium of adenomyosis, there was no significant difference in the expression of STAT3 mRNA and protein levels (P＞0.05). VEGF immunostaining mainly localized in the the glandular epithelial cells. In ectopic endometrium of adenomyosis, the expression of VEGF was significantly higher than the controls（P＜0.05). There was no significant difference in the expression of VEGF in the proliferative phase and secretory phase of each group (P＞0.05). Conclusion p-STAT3 (Y705) may play a role via promoting angiogenesis in the development of the adenomyosis.
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