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活化白细胞黏附分子、E-钙黏蛋白和β-连环蛋白在甲状腺乳头状癌中的表达及意义
Expression and mechanism of activated leukocyte cell adhesion molecule, E-cadherin and β-catenin in papillary thyroid carcinoma
目的 通过对甲状腺乳头状癌（PTC)、结节性甲状腺肿和癌旁正常组织中活化白细胞黏附分子（ALCAM）、E-钙黏蛋白（E-cadherin）和β-连环蛋白（β-catenin）蛋白表达水平的检测，分析三者在PTC发生、发展过程中的作用及三者之间的相关性，探讨ALCAM、E-cadherin和β-catenin在PTC中的作用机制。 方法 采用免疫组织化学法、Western blotting法检测ALCAM、E-cadherin和β-catenin在PTC中的表达并分析三者的变化与临床病理特征的关系。 结果 PTC组织中ALCAM的阳性率明显高于结节性甲状腺肿和癌旁正常组织（P＜0.05）；PTC组织中E-cadherin的阳性表达率明显低于结节性甲状腺肿和癌旁正常组织（P＜0.05）；PTC样本中β-catenin的异位表达率明显高于结节性甲状腺肿和癌旁正常组织（P＜0.05）。三者的阳性率在结节性甲状腺肿和癌旁正常组织中无明显差异（P＞0.05）。三者的异常表达与PTC淋巴结转移有关（P＜0.05），与患者年龄、性别、肿瘤大小无关（P＞0.05）。 结论 在PTC组织中ALCAM、β-catenin高表达，E-cadherin呈现低表达，三者的异常表达与淋巴结转移相关。三者在PTC的发生、发展和转移的过程中起重要的作用。
Objective To detect the expression of activated leukocyte cell adhesion molecule (ALCAM), E-cadherinand β-catenin in the development of papillary thyroid carcinoma（PTC）,nodular goiter（NG）and healthy persons and to explore the mechanisms of ALCAM, E-cadherin and β-catenin in PTC. Methods The expressions of ALCAM,E-cadherin and β-catenin proteins in PTC were detected by Western blotting and immunohistochemical SP method, and the relationships with the clinical pathology characteristics were analyzed. Results The positive expression of ALCAM in the tissue of PTC was significantly higher than that in the normal tissue adjacent to carcinoma and in the tissue of NG(P＜0.05). The positive expression of E-cadherin in the tissue of PTC was significantly lower than that in the normal tissue adjacent to carcinoma and in the tissue of NG(P＜0.05). The ectopic expression rate of β-catenin in the tissue of PTC was significantly higher than in the tissue of NG and the normal tissue adjacent to carcinoma (P＜0.05). The expression of ALCAM, E-cadherin and β-catenin in the tissue of nodular goiter was not different from the normal tissue adjacent to carcinoma(P＞0.05). The abnormal expressions of ALCAM, E-cadherin and β-catenin were unrelated to the age, sex and size of the tumor (P>0.05) of PTC patients, but associated with the lymph node metastasis (P<0.05).
Conclusion The expressions of ALCAM and β-catenin are stronger in PTC tissues than in NG. E-cadherin was little expression in the tissue of PTC. The abnormal expressions of ALCAM, E-cadherin and β-catenin have the positive correlation with the lymph node metastasis.They play an important role in the process of PTC, including the occurrence, development,invision and metastasis.
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河北省教育厅青年基金;河北省卫生厅医学研究课题;河北省重大医学科研课题;河北北方学院青年基金项目
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