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小鼠大脑皮质发育过程中神经细胞、胶质细胞及血管之间的相互关系
Relationship between nerve cells, glial cells and blood vessels during the development of mouse cerebral cortex
目的 探讨小鼠大脑皮质发育过程中神经细胞、胶质细胞及血管发生之间的关系。方法 应用免疫荧光、5-溴脱氧尿嘧啶核苷（BrdU）和墨汁血管灌注等技术，对胚胎受孕15d(E15)至生后90d（P90）小鼠大脑内神经干细胞、神经前体细胞、神经胶质细胞和血管进行形态学观察。 结果 E15左右大脑皮质内开始出现血管网，随着年龄的增长血管分支逐渐增多，血管体密度呈现增长趋势；成年后（P60）血管密度基本稳定。大脑皮质部位小血管走行方向与放射状胶质细胞长突起延伸方向一致，呈平行分布；大量放射状胶质细胞伸出膨大的足板参与血脑屏障的构筑；增殖的神经干细胞主要沿着血管走行方向及放射状胶质细胞长突起延伸方向进行迁移。 结论 在大脑皮质发育过程中，神经干细胞不断增殖，并沿着血管走行方向及放射状胶质细胞长突起延伸方向进行迁移。神经细胞、神经胶质细胞参与血管壁的构成，三者之间相互诱导，共同维持血脑屏障的完整与功能调节。
Objective To investigate the relationship between nerve cells, glial cells and angiogenesis in mouse cerebral cortex development. Methods Brain neural stem cells, neural precursor cells, glial cells and blood vessels of embryonic 15 days (E15) to postnatal 90 days (P90) mice were labeled by immunofluorescence, 5-bromodeoxyuridine(BrdU) and ink perfusion for morphological observation. Results Around the embryonic period of E15, the cerebral cortex began to have vascular networks, and later with the growth of age, the vascular branches gradually increased, the density of the blood vessels showed an increasing trend, and the density of adult (P60) blood vessels was stable. The direction of the small blood vessels in the cerebral cortex was consistent with that of radial glial cells. A large number of radial glial cells extended enlargement of the footplate participating in construction of the blood brain barrier. The proliferation of neural stem cells migrated along the direction of the vessel and the extension direction of radial glial cells. Conclusion During the development of cerebral cortex, neural stem cells proliferate and migrate along the direction of blood vessels and radial glial cells. Nerve cells and glial cells are involved in the structure of the vessel wall, and the interaction among them is to maintain the integrity and function of the blood brain barrier.
［1］Larrivee B, Freitas C, Suchting S, et al. Guidance of vascular development: lessons from the nervous system［J］. Circ Res, 2009, 104(4):428-441.
［2］Zhong Z, Deane R, Ali Z, et al. ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration［J］. Nat Neurosci, 2008, 11(4):420-422.
［3］Banisadr G,Bhattacharyya BJ,Belmadani A, et al. The chemokine BRAK/CXCL14 regulates synaptic transmission in the adult mouse dentate gyrus stem cell niche［J］. J Neurochem,2011, 119(6):1173-1182.
［4］Paez-Gonzalez P, Abdi K, Luciano D, et al. Ank3-dependent SVZ niche assembly is required for the continued production of new neurons［J］.Neuron,2011,71(1):61-75.
［5］Decimo I, Bifari F, Krampera M, et al. Neural stem cell niches in health and diseases［J］.Curr Pharm Des, 2012,18(13):1755-1783.
［6］Niu BH, Zhang Y, Niu YL, et al. The development of radial glial cells and cerebellar cortex of mice［J］. Acta Anatomica Sinica, 2010,41(4):498-503.(in Chinese)
牛保华，张艳，牛艳丽，等. 小鼠放射状胶质细胞和小脑皮质的发育［J］. 解剖学报，2010, 41(4)：498-503.
［7］Riquelme PA, Drapeau E, Doetsch F. Brain micro-ecologies: neural stem cell niches in the adult mammalian brain［J］. Philos Trans R Soc Lond B Biol Sci,2008,363(1489):123-137.
［8］Gómez-Gaviro MV, Lovell-Badge R, Fernández-Avilés F, et al. The vascular stem cell niche［J］. J Cardiovasc Transl Res, 2012,5(5):618-630.
［9］Goldman SA,Chen Z. Perivascular instruction of cell genesis and fate in the adult brain［J］. Nat Neurosci, 2011,14(11):1382-1389.
［10］Li D, Takeda N, Jain R, et al. Hopx distinguishes hippocampal from lateral ventricle neural stem cells ［J］. Stem Cell Res, 2015,15(3):522-529.
［11］Serlin Y, Shelef I, Knyazer B, et al. Anatomy and physiology of the blood-brain barrier ［J］. Semin Cell Dev Biol, 2015,38:2-6.
［12］Prasitsak T, Nandar M, Okuhara S, et al. Foxc1 is required for early stage telencephalic vascular development ［J］. Dev Dyn, 2015,244(5):703-711.
［13］Martoncikova M, Fabianova K, Schreiberova A, et al. Astrocytic and vascular scaffolding for neuroblast migration in the rostral migratory stream［J］. Curr Neurovasc Res,2014,11(4):321-329.
［14］Yafai Y,Eichler W,Iandiev I, et al. Thrombospondin-1 is produced by retinal glial cells and inhibits the growth of vascular endothelial cells［J］. Ophthalmic Res, 2014,52(2):81-88.
［15］Shen Q, Goderie SK, Jin L, et al. Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells［J］. Science,2004, 304(5675):1338-1340.
［16］Peretto P, Giachino C, Aimar P, et al. Chain formation and glial tube assembly in the shift from neonatal to adult subventricularzone of the rodent forebrain［J］. J Comp Neurol,2005, 487(4):407-427.
［17］Palmer TD, Willhoite AR,Gage FH. Vascular niche for adult hippocampal neurogenesis［J］. J Comp Neurol,2000,425(4):479-494.
［18］Gomez-Gaviro MV, Lovell-Badge R, Fernandez-Aviles F, et al. The vascular stem cell niche［J］. J Cardiovasc Transl Res, 2012,5(5):618-630.
［19］Goldman SA, Chen Z. Perivascular instruction of cell genesis and fate in the adult brain［J］. Nat Neurosci, 2011,14(11):1382-1389.
［20］Ottone C, Parrinello S. Multifaceted control of adult SVZ neurogenesis by the vascular niche［J］. Cell Cycle,2015,14(14):2222-2225.
［21］Ma S, Kwon HJ, Johng H, et al. Radial glial neural progenitors regulate nascent brain vascular network stabilization via inhibition of Wnt signaling［J］. PLoS Biol,2013,11(1):e1001469.
［22］Ramirez-Castillejo C, Sánchez-Sánchez F, Andreu-Agulló C, et al. Pigment epithelium-derived factor is a niche signal for neural stem cell renewal ［J］. Nat Neurosci, 2006, 9(3):331-339.
［23］Bostr?m M, Hellstr?m Erkenstam N, Kaluza D, et al. The hippocampal neurovascular niche during normal development and after irradiation to the juvenile mouse brain ［J］. Int J Radiat Biol,2014,90(9):778-789.
［24］Wang L,Chopp M,Gregg SR,et al. Neural progenitor cells treated with EPO induce angiogenesis through the production of VEGF［J］. J Cereb Blood Flow Metab,2008, 28(7):1361-1368.
［25］Folkins C,Man S, Xu P, et al. Anticancer therapies combining antiangiogenic and tumor cell cytotoxic effects reduce the tumor stem-like cell fraction in glioma xenograft tumors［J］. Cancer Res, 2007, 67(8):3560-564.
［26］Sylma D, Xavier M. Gliomagenesis and neural stem cells：key role of hypoxia and concept of tumor“neo-niche”［J］.Medical Hypotheses, 2008, 70(1):96-104.
［27］Veeravagu A, Bababeygy SR, Kalani MY, et al. The cancer stem cell-vascular complex brain tumor formation［J］.Stem Cells Dev, 2008, 17(5):859-867.
［28］Sun Y, Jin K, Childs JT, et al. Vascular endothelial growth factor-B (VEGF-B) stimulates neurogenesis: evidence from knockout mice and growth factor administration［J］. Dev Biol, 2006, 289(2):329-335.
［29］Iadecola C,Nedergaard M. Glial regulation of the cerebral microvasculatur［J］. Nat Neurosci, 2007, 10(11):1369-1376.
中国国家自然科学基金面上项目;中国国家自然科学基金青年基金
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