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小鼠回肠血管透明标本制作方法比较
Comparison of methods for preparing mouse ileum vascular transparent specimen
目的 探讨运用不同填充剂与透明方式制作小鼠回肠血管透明标本的方法，评价其优缺点。方法 运用简易毛细管黏滞计测定填充剂的黏滞系数。8只小鼠被分为4组。采用改良灌流方法清洗血管，分别注入不同的填充剂，用不同的透明方法处理，比较肠组织和透明剂的折光指数。结果 硝化纤维素填充剂的黏滞系数、填充效果均优于其他组；20℃下，肠组织与透明剂折光指数差值分别为甘油法: 2.2×10-3 nD，透明-固定液法: 65.8×10-3 nD。结论 以硝化纤维素填充剂填充血管，使用透明固定液法处理后，得到的标本效果较好。
Objective To compare the advantages and disadvantages of the method for preparing mouse ileum transparent vascular specimens with different fillers and transparent manner. Methods The viscosity coefficients(VCs) of the filler through a simple capillary tube were measured. Eight mice were divided into 4 groups. The blood vessel was washed by the modified perfusion method, injected with different fillers, and treated with different transparency. The refractive indexes(RIs) of ileum tissues and transparent agent were compared. Results Both of the VCs and filling effect of material nitrocellulose were better than other groups. At 20℃, the difference value of RIs between ileum tissues and transparent solutions were 2.2×10-3 nD (Glycerin) and 65.8×10-3 nD (Transparent-fixative), respectively.Conclusion The specimen which was filled with nitrocellulose and treated with transparentfixative method has the better effect.
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