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乳腺癌患者原发磷酸化信号转导与转录激活因子3活性和白细胞介素-17表达的临床意义
王菊 张楠 马志萍 李一鑫 张文杰* 周宗瑶*
解剖学报 ›› 2015, Vol. 46 ›› Issue (6) : 791-796.
乳腺癌患者原发磷酸化信号转导与转录激活因子3活性和白细胞介素-17表达的临床意义
Clinical significance of phosphorylated signal transducer and activator of transcription 3 activing and interleukin-17 in patients with breast cancer
目的 探讨炎性信号通路磷酸化信号转导和转录激活因子3（p-STAT）的原发活化状态及下游细胞因子白细胞介素（IL）-17表达在乳腺癌发生和发展中的作用及其对预后的影响。 方法 运用免疫组织化学Envision两步法检测了379例乳腺癌患者以及匹配癌旁245例乳腺腺病中IL-17表达和原发性p-STAT3的活化状态，分析了它们与乳腺癌组织学分型、TNM分期、临床分期及预后的相关性。统计学分析：计量数据使用t检验法，计数数据使用χ2 检验法。结果 1. IL-17在乳腺癌中的阳性表达率为95.8%，显著高于乳腺腺病中的阳性表达率85.3%（χ2=21.363，P<0.001）；具有活性的p-STAT3在乳腺癌中的阳性率为93.6%，也显著高于乳腺腺病中的阳性率62.0%（χ2=97.702，P<0.001）。2. IL-17和p-STAT3在乳腺癌 (包括乳腺浸润性导管癌、乳腺浸润性小叶癌和腺导管原位癌分型) 中的阳性率与乳腺癌组织学分型无相关性（χ2=1.245, P=0.535＞0.05）。3. IL-17和p-STAT3在乳腺癌中的强阳性率分别与淋巴结的转移成正相关（IL-17: χ2=7.806, P<0.01; p-STAT3: χ2=4.053, P<0.05）。4. 在乳腺癌组织中，IL-17表达与p-STAT3活性呈正相关（rs=0.136, P<0.01）。 结论 原发增高的p-STAT3活性及高表达的炎性细胞因子IL-17可能协同作用，产生炎性微环境，从而参与乳腺癌的发生和发展。
Objective To investigate involvement of constitutively activated phosphorylated signal transducer and activator of transcription 3(p-STAT3) and the expression of its downstream inflammatory cytokine interleukin (IL)-17 in the pathogenesis and progression in patients with breast cancer(BC). Methods Using specific antibodies, the levels of IL-17 and p-STAT3 were tested in 379 cases of breast cancer tissues (test group) and 245 cases of matching non-cancerous adjacent breast adinosis tissues (control group) using immunohistochemistry (IHC). The correlations of expressed IL-17 and active p-STAT3 were analyzed against breast cancer patients’ histological type, TNM and clinical stage, and progression, respectively. Quantitative data were analyzed using t test and qualitative data were analyzed using χ2 test. Results 1. IL-17 showed a positive expression rate of 95.8% in brest cancer tissues, higher than that of 85.3% in adenosis of breast tissues (χ2=21.36, P＜0.001); p-STAT3 exhibited a positive expression rate of 93.6% in BC tissues, much higher than that of 62.0% in adenosis of breast tissues (χ2=97.7，P<0.001). 2. The expressed IL-17 and active p-STAT3 were not correlated with histologic types of breast cancer (including invasive ductual carcinoma, invasive lobulao carcinoma, ductual carcinoma in situof breast) (χ2=1.245, P=0.535). 3. However, IL-17 and p-STAT3 were respectively correlated with lymph node metastasis in patients with BC (χ2=7.806, P<0.01; χ2=4.053, P<0.05, respectively). 4. In breast cancer tissues, the expression of IL-17 was positively correlated with activated p-STAT ( rs=0.136, P<0.01). Conclusion The constitutively over-active p-STAT3 and over-expressed IL-17 may collaborate to orchestrate an inflammatory microenvironment which, in turn, facilitates the pathogenesis and progression of breast cancer.
白细胞介素-17 / 信号转导和转录激活因子3 / 乳腺癌 / 癌转移 / 免疫组织化学 / 人
Interleukin-17 / Phosphorylated signal transducer and activator of transcription 3 / Breast cancer / Metastasis / Immunohistochemistry / Human
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