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Axl及配体Gas6在甲状腺乳头状癌中的表达及意义
Expression and significance of receptor tyrosine kinase Axl and its ligand Gas6 in thyroid papillary carcinoma
目的 通过检测Axl、Gas6在甲状腺乳头状癌（PTC）中的表达, 分析和探讨两者在甲状腺乳头状癌发生、发展中的作用及相关性。方法 采用免疫组织化学法、Western blotting及Real-time PCR法检测Axl、Gas6在甲状腺乳头状癌中的表达并分析两者的变化与临床病理特征的关系。结果 与结节性甲状腺肿（NG）及瘤旁正常组织相比，甲状腺乳头状癌组织中Axl、Gas6的mRNA及蛋白表达水平明显升高（P＜0.05），且蛋白表达量与淋巴结转移、临床病理分期有关，与患者年龄、性别、肿瘤大小无关。 结论 Axl和 Gas6两者在PTC中的表达量高于结节性甲状腺肿。在PTC中Axl、Gas6的高表达与肿瘤的淋巴结转移和临床病理分期呈正相关，两者在PTC的发生、发展、转移的过程中起重要的作用。
Objective To analyze the role and correlation of Axl and Gas6 during the development of papillary thyroid carcinoma (PTC) by detecting the expressions of Axl and Gas6 in the PTC tissues. Methods The expressions of Axl and Gas6 proteins in PTC were detected by Western blotting and immunohistochemical SP method, and the relationships with the clinical pathology characteristics were analyzed. The transcriptional level changes of Axl mRNA and Gas6 mRNA were tested by Real-time reverse transcription polymerase chain reaction (Real-time PCR). Results 1. The results of immunohistochemistry staining showed Axl proteins were expressed in different strength in all PTC tissues. Axl positive proteins were mainly located in the cancer cell membrane, A few were found in the cytoplasm. In nodular goiter(NG) and normal tissues adjacent to the tumors Axl expression was weakly positive or negative.Gas6 was mainly expressed in PTC cells cytoplasm, and negative expression in NG and normal tissues adjacent to the tumors. Axl and Gas6 positive expression rate in PTC was significantly higher than in NG and normal tissues adjacent to the tumors（P＜0.01）whose expressions were no significant difference between in NG and normal tissues adjacent to the tumors（P＞0.05）. The abnormal expressions of Axl and Gas6 were unrelated to the age, sex and size of the tumor (P>0.05) of PTC patients, but associated with the lymph node metastasis and pathological stage (P<0.05). 2. The results of Western blotting showed the Axl and Gas6 proteins in PTC tissues were significantly more than in NG and normal tissues adjacent to the tumors with statistical significance. (P<0.05), but no statistical difference between in NG and normal tissues adjacent to the tumors (P>0.05).3. The results of Real-time PCR showed the transcriptional expression levels of the Axl mRNA and Gas6 mRNA in PTC tissues were significantly higher than in NG and normal tissues adjacent to the tumors with statistical significance(P<0.05), but no statistical difference between in NG and normal tissues adjacent to the tumors (P>0.05). Conclusion Axl and Gas6 exist in PTC and NG tissues,and their expressons are stronger in PTC tissues than in NG.The higher expressions of Axl and Gas6 have the positive correlation with the lymph node metastasis and clinicopathologic stage. Axl and Gas6 play an important role in the process of PTC,including the occurrence, development,invision and metastasis.
Axl / Gas6 / 甲状腺乳头状癌 / 免疫印迹法 / 实时定量聚合酶链反应 / 人
Anexelekto receptor tyrosine kinase / Growth arrest specific gene 6 / Thyroid papillary carcinoma / Western blotting / Real-time PCR / Human
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河北省卫生厅医学研究课题;河北省重大医学课题;河北北方学院青年基金项目
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