氧化损伤对骨髓源性肝前体细胞恶性转化的影响

文朝阳 史争鸣 赵娜 薛晓伟 丰平 王学江*

doi:10.16098/j.issn.0529-1356.2015.06.009

解剖学报 ›› 2015, Vol. 46 ›› Issue (6) : 780-784. DOI: 10.16098/j.issn.0529-1356.2015.06.009

肿瘤生物学

氧化损伤对骨髓源性肝前体细胞恶性转化的影响

文朝阳¹史争鸣²赵娜³ 薛晓伟³ 丰平³ 王学江^3*

作者信息 +

Effect of oxidative damage on malignant transformation of bone marrow-derived hepatic progenitor cells

WEN Zhao-yang¹ SHI Zheng-ming²ZHAO Na³XUE Xiao-wei³ FENG Ping³ WANG Xue-jiang ^3*

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文章历史 +

摘要

目的探讨活性氧自由基对骨髓源性肝前体细胞恶性转化的影响。方法取经过鉴定的3代骨髓间充质干细胞（BMSCs）分为3组：对照组（BMSCs），生长因子诱导组（BMSCs+HGF+EGF），过氧化氢刺激组（BMSCs+HGF+EGF+ H₂O₂）。分别在细胞培养的第7天、14天、21天、28天用RT-PCR方法检测各组细胞的白蛋白(ALB)、甲胎蛋白（AFP）、角蛋白18 (CK18) mRNA的表达。用免疫细胞化学方法检测细胞γ-谷氨酰转酞酶(γ-GT)的表达，用Schiff染色方法检测细胞DNA含量，用流式细胞仪检测非整倍体细胞。结果生长因子诱导组及过氧化氢刺激组细胞有ALB mRNA、AFP mRNA及CK18 mRNA的表达，过氧化氢刺激组细胞AFP mRNA、CK18 mRNA、γ-GT的表达、DNA含量及非整倍体细胞均高于生长因子诱导组（P<0.05）。结论骨髓间充质干细胞在体外诱导成为肝前体细胞，活性氧自由基可促使肝前体细胞过度增殖并恶性转化。

Abstract

Objective To explore the role of oxidative damage in malignant transformation of bone marrow-derived liver stem cells. Methods Bone marrow mesenchymal stem cells (BMSCs) were divided into three groups: control group（BMSCs）, GF induction group（BMSCs+GF）and Hydrogen peroxide stimulate group（BMSCs+GF+ H₂O₂). Albumin (ALB) mRNA, alpha-fetoprotein (AFP) mRNA and CK18 mRNA were detected by RT-PCR at 7d, 14d, 21d and 28d. Gamma glutamyl transpeptidase (γ-GT) was detected by immunocytochemical technique. DNA contents were detected by Schiff dyeing. Aneuploid somatic cells were detected by flow cytometry. Results The results of in vitro experiments showed that there were expressions of ALB mRNA, AFP mRNA and CK18mRNA in group BMSCs+GF and group BMSCs+GF+ H₂O₂. The expressions of AFP mRNA, CK18mRNA, γ-GT, DNA contents and aneuploid somatic cells in group BMSCs+GF+ H₂O₂ were higher than that in group BMSCs+GF. Conclusion Reactive oxygen species can induce bone marrow mesenchymal stem cell proliferation and malignant transformation.

导出引用

文朝阳史争鸣赵娜薛晓伟丰平王学江*. 氧化损伤对骨髓源性肝前体细胞恶性转化的影响[J]. 解剖学报. 2015, 46(6): 780-784 https://doi.org/10.16098/j.issn.0529-1356.2015.06.009

WEN Zhao-yang SHI Zheng-ming ZHAO Na XUE Xiao-wei FENG Ping WANG Xue-jiang*. Effect of oxidative damage on malignant transformation of bone marrow-derived hepatic progenitor cells[J]. Acta Anatomica Sinica. 2015, 46(6): 780-784 https://doi.org/10.16098/j.issn.0529-1356.2015.06.009

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