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黄芪(粗)多糖对麻黄素损伤小鼠肾组织结构和抗氧化物酶活性及Caspase-3蛋白表达的影响
魏娉婷 李重阳 俞诗源*孟茹袁静
解剖学报 ›› 2015, Vol. 46 ›› Issue (4) : 564-571.
黄芪(粗)多糖对麻黄素损伤小鼠肾组织结构和抗氧化物酶活性及Caspase-3蛋白表达的影响
Effect of astragalus polysaccharide on histological structure, enzyme activity and Caspase-3 protein expression in the kidney of mice injured by ephedrine
目的 探讨黄芪（粗）多糖（APS）对麻黄素致损伤小鼠肾组织结构和抗氧化物酶活性的影响。方法 72只昆明小鼠分为空白对照组、实验对照组和黄芪(粗)多糖1、2组。实验对照组和黄芪(粗)多糖1、2组连续腹腔注射0.2 ml浓度为4.0g/L麻黄素溶液，黄芪(粗)多糖1、2组在腹腔注射麻黄素溶液1 h后，分别灌胃0.3 ml(12.5 g/L、25 g/L)APS溶液，空白对照组注射和灌胃等量的生理盐水。用比色法测定小鼠血浆尿素氮（BUN）及肌酐(Cr)含量、肾组织超氧化物歧化酶 (SOD)活性及丙二醛(MDA)含量的变化，用显微镜观察小鼠肾组织结构的变化，用免疫组织化学法及Western blotting法检测肾组织Caspase-3蛋白表达的变化。结果 实验对照组小鼠血浆BUN及Cr含量高于空白对照组，肾组织SOD活性低于空白对照组，MDA含量高于空白对照组 (P<0.05或P<0.01)，肾小球严重肿胀，肾小囊腔狭窄，肾小管上皮细胞有不同程度肿胀、坏死，肾组织Caspase-3蛋白表达高于空白对照组(P<0.05或P<0.01)。与实验对照组相比，APS组小鼠血浆BUN及Cr含量降低，肾组织SOD活性升高，MDA含量降低(P<0.05或P<0.01)，肾小球肿胀显著减轻，肾小囊腔明显可见，肾小管肿胀明显减轻，Caspase-3蛋白表达显著降低(P<0.05或P<0.01)。 结论 APS能提高小鼠肾功能及肾组织细胞的抗氧化酶活性，能降低肾组织Caspase-3蛋白的表达，可有效减轻麻黄素对小鼠肾组织的损伤，对麻黄素致损伤小鼠有明显的保护效应。
Objective To investigate astragalus polysaccharide（APS）on histological structure and enzyme activity in kidney of mice injured by ephedrine. Methods Seventy-two Kunming mice were divided into 4 groups：the nature control group, the experimental control group,the APS1 and 2 groups. The experimental control group, and APS1 and 2 groups were intraperitoneally injected with 4.0 g/L of ephedrine, 0.2ml twice a day for 15 days. The APS1 and 2 groups were respectively intragastric ally administed with 0.3 ml APS (12.5 g/L,25g/L) 1 hour after ephedrine injection, the control group was injected and intragastric ally administed with the same amount of saline.The blood urea nitrogen (BUN) and creatinine(Cr) content of plasma as well as the activity of superoxide dismutase (SOD) and the malondialdehyde (MDA) content of kidney tissue in the kidney were determined by colorimetry. The kidney histological structures were observed by optical microscopy. The changes of Caspase-3 protein expression in kidney were detected by immunohistochemistry and Western blotting. Results The BUN and Cr content in the blood of the experimental control groups were higher than that of the nature control group. The SOD activity in kidney of the experimental control groups was lower than the nature control groups, while the content of MDA was higher than the nature control groups (P<0.05 or P< 0.01).The histological structure lesions of the experimental control group showed that the glomerular swelled seriously, the renal capsule lumen stenosis,the swelling and necrosis of the renal tubular epithelial cells accured in different degrees. The expressions of Caspase-3 protein in kidney of the experimental control groups were higher than the control groups (P<0.05 or P<0.01). The BUN and Cr content of plasma of the APS groups were lower than the experimental control groups. The SOD activity in the renal tissue of the APS groups increased,while the MDA content decreased (P<0.05 or P<0.01). The histological structure lesions of the APS groups were relieved, the renal capsule lumen were obvious, the swelling of the renal tubular reduced, the degeneration and exfoliating of the epithelial cells alleviated. The expression of Caspase-3 protein in kidney declined significantly (P<0.05 or P<0.01). Conclusion APS enhanced the cells antioxidant enzyme activity, and reduced the expression of Caspase-3 protein.Therefore, APS alleviated the damage of kidney tissue caused by ephedrine effectively, and significantly protected the mice form the ephedrine effects.
黄芪(粗)多糖 / 麻黄素 / 肾功能 / 组织结构 / 超氧化物歧化酶 / 比色法 / 免疫印迹法 / 小鼠
Astragalus polysaccharide / Ephedrine / Renal function / Histological structure / Superoxide dismutase / Colorimetric method / Western blotting / Mouse
［1］Zhou Y, Bunney BS, Shi WX. Differential effect of cocaine on firing rate and pattern of dopamine neurons role of alphal receptor sand comparison with L-Dopa and apomorphine ［J］. J Pharmacol Exp Ther, 2006, 317(1):196-201.
［2］Marie LB. Fulminant exacerbation of autoimmune hepatitis after the use of Ma Huang ［J］. Am J Gastroenterol, 2001, 9(5)：1654-1655.
［3］Nadir A, Agarwal S, King PD. Acute hepatitis associated with the use of a Chinese herbal product, Ma-huang ［J］. Am J Gastroenterol, 1996, 91（7）: 1436-1438.
［4］Yu ShY, Liu XY, Wu ZhZ, et al. Protection of Chinese compound medicine on histological structure of ovarian in filial mice of ephedrine injury［J］. Journal of Northwest Normal University, 2014, 50(3):79-83. (in Chinese)
俞诗源，刘小媛，吴转子，等.中药复方液对麻黄素损伤小鼠卵巢的保护作用［J］. 西北师范大学学报，2014, 50(3):79-83.
［5］Yang ZhX, Wang Q. Progress of basic and clinical study on immunization of APS ［J］. World of Traditional Chinese Medicine, 2013, 8(7): 833-838. (in Chinese)
杨志霞，王琦. 黄芪多糖免疫作用的基础与临床研究进展［J］.世界中医药，2013, 8(7)：833-838.
［6］Sun RM, Liu DP, Chen J, et al. Extraction of Astragalus polysaccharide in Astragalus［J］. Acta Scientiarum Naturalium Universitatis Nankaiensis, 2005, 38(1)：33-36. (in Chinese)
孙瑞敏，刘冬平，陈俊，等. 黄芪多糖的提取［J］. 南开大学学报（自然科学版），2005,38(1): 33-36.
［7］Sun J, Li ChY, Yu ShY, et al.Effect on liver tissue structure and related active substances after injecting ephedrine of pups［J］. Acta Anatomica Sinica, 2012, 43(3): 398-405. (in Chinese)
孙隽，李重阳，俞诗源，等.麻黄素对仔鼠肝脏组织结构和相关活性物质的影响［J］.解剖学报，2012, 43(3): 398-405.
［8］Li L, Yu ShY, Ma JB, et al. Effects of melamine on histological structure and activities of superoxide dismutase,catalase and content of maleic dialdehyde in kidney of filial mice［J］. Acta Anatomica Sinica, 2011, 42(1): 91-95. (in Chinese)
李丽，俞诗源，马建博，等.三聚氰胺对仔鼠肾脏组织结构和超氧化物歧化酶、过氧化氢酶活性及丙二醛含量的影响［J］.解剖学报，2011，42.(1): 91-95.
［9］Wang JJ, Li ChY, Yu ShY, et al.Ephedrine for pups kidney structure and SOD, CAT, and MDA content［J］. Chinese Journal of Zoology, 2011, 46(6)：118-125. (in Chinese)
王锦锦，李重阳，俞诗源，等.麻黄素对仔鼠肾组织结构和SOD，CAT及MDA含量的影响［J］.动物学杂志，2011, 46(6): 118-125.
［10］Jin D, Liang Y, Zheng WF, et al. Antioxidant activity of polysaccharides from scutellaria baicalensis georgi in vitro［J］.Veterinary Medicine Magazine, 2012, (3): 34-37. (in Chinese)
金迪，梁英，郑文凤，等. 黄芪多糖体外抗氧化活性研究［J］. 中兽医医药杂志，2012, (3): 34-37.
［11］Yu ShY, Wei PT, Guo Y, et al. The effect of Chinese medicine compound on content of serum BUN, Cr and kidney tissue of filial mice injured by ephedrine ［J］. Journal of Northwest Normal University, 2013, 49(6): 84-88. (in Chinese)
俞诗源，魏娉婷，郭耀，等. 中药复方对麻黄素仔鼠血清BUN、Cr含量和肾组织的影响［J］.西北师范大学学报，2013, 49(6): 84-88.
［12］Mao ShM, Li ChD, Li JJ, et al. Effects of Astragalus polysaccharide on the renal injury of diabetes rats and renal expression of aquaporor-2［J］. China Journal of Gerontology, 2010, 8(30)：2301-2305. (in Chinese)
毛淑梅，李承德，李静静. 黄芪多糖对糖尿病大鼠肾脏水通道蛋白2表达的影响及对肾脏的保护作用［J］. 中国老年学杂志，2010, 8(30): 2301-2305.
［13］Li L, Yu L, Li N, et al. Astragalus mongholicus and Angelica sinensis compound alleviates nephrotic hyperlipidemia in rats ［J］. Chin Med J(Eng 1), 2000, 113(4)：310-314.
［14］Zhang YW, Wu CY, Chen JT. Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expression of NF-κB and IκB in renal cortex of streptozotoxininduced diabetic rats ［J］. J Ethonpharmacol, 2007, 114(3)：387-392.
［15］Li HQ, Duan XP, Ma HL, et al. Effect of APS on immunization by increasing antioxidation in chickens ［J］. Journal of Shanxi Agriculture University, 2002, 22(1): 78-81. (in Chinese)
李宏全，段县平，马海丽，等. 黄芪多糖提高鸡抗氧化作用对免疫功能的影响［J］. 山西农业大学学报，2002, 22(1): 78-81.
［16］Kumar S. Caspase function in programmed cell death ［J］. Cell Death Differ, 2007, 14(1): 32-43.
［17］Wang YL, Wang ZhW, Wang XJ, et al.Caspase-3 gene of mediating X radiation induced apoptosis in nasopharyngeal carcinoma［J］. Cancer Prevention Research, 2009, 36(7): 541-545. (in Chinese)
王亚利，王中卫，王西京，等. Caspase-3在X线诱导的鼻咽癌细胞凋亡中的调控作用肿瘤防治研究［J］. 肿瘤防治研究，2009, 36(7): 541-545.
［18］Mazumder S, Plesca D, Almasan A,et al.Caspase-3 activation is a critical determinant of genotoxic stressinduced apoptosis［J］. Methods Mol Biol, 2008, 414: 13-21.
［19］Kuribayashi K, Mayes PA, El-Deiry WS. Journal club what are Caspase 3 and 7 doing upstream of the mitochondria ［J］ ? Cancer Biol Ther, 2006, 5(7): 763-765.
［20］Jonges LE, Nagelkerke JF, Emink NG, et al.Caspase-3 activity as a prognostic factor in colorectal carcinoma［J］. Lab Invest, 2006, 81(1): 681-688.
［21］Yang NSh, Huang YF, Ye R, et al. Cell proliferation and apoptosis in the rat crescentic glomerulonephritis ［J］. Chinese Journal of Nephrology, 1998, 14(3)：147-150. (in Chinese)
杨念生，黄越芳，叶任，等.大鼠新月体肾炎中的细胞增殖与凋亡［J］. 中华肾病杂志，1998, 14(3): 147-150.
［22］Wei X, Zhang J, Li J, et al. Astragalus mongholicus and Polygonum multiflorum’s protective function against cyclophosphamide inhibitory effect on thymus［J］. Am J Chin Med, 2004, 32(5): 669-680.
［23］Shao P, Zhao LH, Zhi C, et al. Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides［J］. Int Immunopharmacol, 2006, 6(7): 1161-1166.
［24］Liu M, Ouyang JP, Wu K, et al. Effect of astragalus polysaccharide on Ser phosphorylation of protein kinase B in skeletal muscle of KKay mice［J］. Medical Journal of Wuhan University, 2006, 27(2): 136-140. (in Chinese)
刘敏，欧阳静萍，吴珂，等.黄芪多糖对KKay小鼠骨骼肌蛋白激酶B丝氨酸磷酸化的影响［J］. 武汉大学学报(医学版)，2006, 27(2): 136-140.
［25］Li S, Zhang Y, Zhao J. Preparation and suppressive effect of Astragalus polysaccharide in glomerulonephritis rats ［J］. Int Immunopharmacol, 2007, 7(1): 23-28.
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