孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响

单智焱 武玢 张玥 薛媛 吴嫣爽 沈星辉 雷蕾 刘忠华*

doi:10.16098/j.issn.0529-1356.2015.04.019

解剖学报 ›› 2015, Vol. 46 ›› Issue (4) : 553-557. DOI: 10.16098/j.issn.0529-1356.2015.04.019

组织学胚胎学发育生物学

孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响

单智焱¹ 武玢¹张玥¹ 薛媛¹ 吴嫣爽¹ 沈星辉¹ 雷蕾¹ 刘忠华^2*

作者信息 +

Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes

SHAN Zhi-yan¹ WU Bin¹ ZHANG Yue¹ XUE Yuan¹ WU Yan-shuang¹ SHEN Xing-hui¹ LEI Lei¹ LIU Zhong-hua ^2*

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文章历史 +

摘要

目的通过诱导多能干细胞（iPSCs）技术重编程孤雌胚胎干细胞，探讨iPSCs技术对孤雌胚胎干细胞的多能性及印记基因的影响。方法从孤雌激活的囊胚中建立了孤雌胚胎干细胞；利用反转录病毒将多能性转录因子转入孤雌胚胎干细胞中，建立孤雌iPS细胞。结果建立的孤雌来源的iPS细胞体内外分化能力与孤雌胚胎干细胞的差别无显著性；Real-time PCR结果显示，孤雌iPS细胞母源印记基因的表达明显高于孤雌胚胎干细胞，父源印记基因表达下降，多能性基因表达升高。
结论 iPSCs技术能影响基因的表达，尤其是印记基因，印记使其更接近于正常受精来源的胚胎干细胞中印记基因水平。

Abstract

Objective To investigate the effect of pluripotency and the expression of imprinted genes on the parthenogenetic embryonic stem cells (PgESCs) by induced pluripotent stem cells (iPSCs) reprogrmming. Methods In our study, we firstly generated the PgESCs from parthenogenetic blastocyst, induced the pluripotent factors into the PgESCs by retrovirus transfection and generated the iPS cells derived from PgESCs. Results It showed no difference in pluripotency and differentiation between PgESCs and PgESC-iPS cells, however, the expression of maternal imprinted genes was significantly increased and the expression of paternal imprinted genes was decreased in PgESC-iPS cells compared to that in PgESCs. Conclusion These results demonstrated the expression of imprinted genes could be modified by iPSCs reprogramming, which may be close to the expression level of imprinted genes in fertilized embronic stem cells.

导出引用

单智焱武玢张玥薛媛吴嫣爽沈星辉雷蕾刘忠华*. 孤雌胚胎干细胞来源的诱导多能干细胞的建立及对印记基因表达的影响[J]. 解剖学报. 2015, 46(4): 553-557 https://doi.org/10.16098/j.issn.0529-1356.2015.04.019

SHAN Zhi-yan WU Bin ZHANG Yue XUE Yuan WU Yan-shuang SHEN Xing-hui LEI Lei LIU Zhong-hua*. Generation of induced pluripotent stem cells derived from parthenogenetic embryonic stem cells and reprogramming of imprinted genes[J]. Acta Anatomica Sinica. 2015, 46(4): 553-557 https://doi.org/10.16098/j.issn.0529-1356.2015.04.019

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