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小鼠精子发生过程中dysbindin-1的表达
Expression of dysbindin-1 during spermatogenesis
目的 探讨精子发生过程中dysbindin-1的表达及dysbindin-1对精子顶体形态的影响。 方法 取生后7d、14d、21d、28d、35d和3月龄的雄性小鼠各3只，用免疫印迹法检测睾丸组织dysbindin-1的表达；以dysbindin-1缺失突变的sdy小鼠为研究对象，收集附睾尾部精子，一部分制备精子涂片，HE染色显示精子形态，用异硫氰酸荧光素-豌豆凝集素（FITC-PSA）和抗精子蛋白56（sp56）单克隆抗体进行荧光染色显示顶体的结构；另一部分采用免疫印迹法检测精子中dysbindin-1的表达。
结果 不同发育阶段小鼠睾丸组织及精子中均只有dysbindin-1A，无dysbindin-1C的表达；sdy小鼠的精子及顶体的形态未见明显异常。 结论 Dysbindin-1A表达于小鼠精子发生的不同时期，dysbindin-1A对精子形态维持不起关键作用。
Objective To test dysbindin-1 expression during spermatogenesis and dysbindin-1 effect on histology of acrosome in mice. Methods Western blotting analysis was applied to detect the expression of dysbindin-1 in testis on day 7,14,21,28 and 35 postpartum and three-month old mice(n=3). The caudal spermatozoa was collected from sdy mice (no dysbindin-1expression), HE staining was performed for sperm morphology, fluorescein isothiocyanate conjugate-pisum sativum agglutinin (FITC-PSA) staining and immunofluorescence staining with antibody against sperm protein 56(sp56) for acrosome on sperm smear. Results Dysbindin-1A, not -1C, was expressed in postnatal testis development and spermatozoa. Mature sperm from sdy caudal epididymis had normal formation. Conclusion Dysbindin-1A, expressed in testis tissue and sperm during spermatogenesis, is not required for maintaining the morphology of sperm.
Dysbindin-1 / 睾丸 / 精子 / 顶体 / 免疫印迹法 / sdy 小鼠 / 小鼠
Dysbindin-1 / Testis / Sperm / Acrosome / Western blotting / sdy mouse / Mouse
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