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膜联蛋白A7在胃癌组织中的表达和意义
Expression of annexin A7 in human gastric carcinomas and the clinic significance
目的 检测膜联蛋白A7（ANXA7）在胃癌组织中的表达改变，探讨胃癌的发生机制。 方法 收集人胃癌组织55例及正常胃组织25例，细胞裂解液裂解组织细胞，蛋白定量试剂盒进行蛋白定量后，Western blotting法和Real-time PCR法分别于蛋白水平和基因转录水平检测ANXA7在胃癌组织和正常胃组织中的表达改变，并用免疫组织化学法对ANXA7在细胞中的定位和表达进行检测。 结果 ANXA7在胃癌中蛋白水平和转录水平的表达均显著增高，与正常组相比差异有显著性（P<0.05）； ANXA7在胃癌细胞中定位于细胞质。 结论 膜联蛋白A7在胃癌中的表达增高有可能是胃癌发生的机制之一。
Objective To dectect the expression of annexin A7 in human gastric carcinoma so as to detect the mechanism. Methods Fifty five gastric carcinoma samples and 25 normal human gastric samples were collected. Each sample was lysed in lysis buffer. Whole protein of the supernatant was estimated by BCA-100 Protein Quantitative Analysis Kit. The expression of annexin A7 in human gastric carcinoma and normal gastric tissue was examined with Western blotting and Real\|time PCR. Immunehistolchemistry was used to detect the expression position of annexin A7 in the gastric carcinoma cells. Results The expression of annexin A7 increased in gastric cancer not only on protein level but also on RNA level when compared with normal gastric tissues, the difference was significant (P<0.05); Annexin A7 mainly expressed in the cytoplasm of the gastric carcinoma cells. Conclusion Expression of annexin A7 is up-regulated in human gastric cancer, and it may involve in the development of gastric cancer.
膜联蛋白A7 / 胃癌 / 免疫印迹法 / 实时定量聚合酶链反应 / 人
Annexin A7 / Gastric cancer / Western blotting / Real-time PCR / Human
［1］Rand JH. The annexinopathies: a new category of diseases［J］. Arch Oncol, 2006, 14 (1-2):23-25.
［2］Srivastava M, Bubendorf L, Srikantan V, et,al. ANX7, a candidate tumor suppressor gene for prostate cancer［J］. Proc Natl Acad Sci USA, 2001,98(8):4575-4580.
［3］Srivastava M1, Torosyan Y, Raffeld M. ANXA7 expression represents hormone-relevant tumor suppression in different cancers［J］. Int J Cancer, 2007,121(12):2628-2636.
［4］Kataoka TR, Ito A, Asada H,et,al. Annexin VII as a novel marker for invasive phenotype of malignant melanoma［J］. Jpn J Cancer Res, 2000, 91(1):75-83.
［5］Srivastava M, Montagna C, Leighton X,et al. Haploinsufficiency of Anx7 tumor suppressor gene and consequent genomic instabilitypromotes tumorigenesis in the Anx7(+/-) mouse［J］.Proc Natl Acad Sci, 2003,100(24):14287-14292.
［6］Hung KS, Howng SL. Prognostic significance of annexin VII expression in glioblastomas multiforme in humans［J］. J Neurosurg， 2003,99(5):886-892.
［7］Leighton X, Srikantan V, Pollard HB. Significant allelic loss of ANX7region (10q21) in hormone receptor negative breast carcinomas［J］. Cancer Lett， 2004,210(2):239-244.
［8］Srivastava M, Bubendorf L, Raffeld M, et al. Prognostic impact of ANX7-GTPase in metastatic and HER2-negative breast cancer patients［J］. Clin Cancer Res， 2004, 10(7):2344-2350.［9］Qazi AS, Sun M, Huang Y, et al. Subcellular proteomics: determination of specific location and expression levels of lymphatic metastasis associated proteins in hepatocellular carcinoma by subcellular fractionation［J］. Biomed Pharmacother， 2011, 65(6):407-416.
［10］Hsu PI, Huang MS, Chen HC,et al. The significance of ANXA7 expression and its correlation with poor cellular differentiation and enhanced metastatic potential of gastric cancer［J］. J Surg Oncol， 2008, 97(7):609-614.
［11］Sun MZ, Liu S, Tang J, et al. Proteomics analysis of two mice hepatocarcinoma ascites syngeneic cell lines with high and low lymph node metastasis rates provide potential protein markers for tumor malignancy attributes to lymphatic metastasis［J］. Proteomics， 2009, 9(12):3285-3302.
［12］Wang XJ, Li X. Influence of annexin A7 low expression on proliferation of HepG2 cells［J］. Acta Anatomica Sinica, 2013,44(5): 656-660.(in Chinese)
王小杰，李欣.膜联蛋白A7低表达对人肝癌HepG2细胞增殖的影响［J］. 解剖学报，2013，44（5）：656-660.
［13］Tian HN, Wang XJ, Chen L, et al. Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells［J］. Acta Anatomica Sinica,2015,46(2): 216-220.(in Chinese)
田焕娜，王小杰，陈龙，等. 膜联蛋白A7低表达对胃癌MGC-803细胞生物学特性的影响［J］.解剖学报，2015,46(2): 216-220.
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