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新生大鼠多器官成纤维细胞nanog蛋白的表达差异
Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat
目的 探讨新生大鼠心、肝、脾、肺、肾和皮肤中成纤维细胞nanog蛋白表达差异及其意义。方法 用免疫组织化学、免疫荧光和体外细胞培养技术，对成纤维细胞进行nanog蛋白和波形蛋白（vimentin）标记，检测并比较新生大鼠不同器官中成纤维细胞nanog蛋白和vimentin表达及共表达特征。 结果 新生大鼠心、肝、脾、肺、肾和皮肤6种器官中均有nanog蛋白和vimentin表达，在每个器官中均有少数成纤维细胞共表达这两种蛋白，共表达率分别为心（10.07±0.77）%、肝（9.97±3.70）%、脾（11.23±2.98）%、肺（15.07±2.87）%、肾（11.75±2.76）%、皮肤（16.14±1.28）%，其中皮肤和肺的共表达率高于心、肝、脾、肾（P<0.05）。 结论 共表达nanog蛋白和vimentin的成纤维细胞可能更具有干细胞样特性；新生大鼠皮肤和肺的成纤维细胞可能更具有组织损伤修复潜能。
Objective To investigate different expression and its significance of nanog protein in multiple organs fibroblasts of neonatal rats, including heart, liver, spleen, lung, kidney and skin. Methods The expresssion and co-expression of nanog and vimentin in fibroblasts were detected in multiple organs by immunohistochemistry and immunofluorescence. Fibroblasts in multiple organs cultured in vitrowere labeled with nanog and vimentin by immunofluorescence. Results Nanog and vimentin were detected in six organs in neonatal rats respectively. Co-expression rates of these two proteins were（10.07±0.77）% in heart,（9.97±3.70）% in liver,（11.23±2.98）% in spleen,（15.07±2.87）% in lung,（11.75±2.76）% in kidney and（16.14±1.28）% in skin. So co-expression rates of skin and lung were higher than that of other four organs（P<0.05）. Conclusion Fibroblasts in multiple organs which can express nanog may have stem cell-like characters and fibroblasts in skin and lung of neonatal rats have more potential for organ repair.
nanog蛋白 / 波形蛋白 / 成纤维细胞 / 免疫组织化学 / 免疫荧光 / 新生大鼠
Nanog / Vimentin / Fibroblast / Immunohistochemistry / Immunofluorescence / Neonatal rat
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