鸵鸟眼球栉膜的形态学观察
Morphologcal study of the pecten oculi from ostrich (Struthio camelus)
目的 观察和分析10只鸵鸟眼球栉膜的解剖学和组织学结构。 方法 采用常规石蜡切片,苏木素-伊红(HE)染色、Gordon-Sweet法、Van Gieson苦味酸酸性复红染色法,对健康的1 0月龄非洲黑颈鸵鸟眼球栉膜的形态学结构进行观察和分析。 结果 鸵鸟眼球栉膜位于视乳头部,呈椭圆锥体形浸入玻璃体内。体视显微镜下可见栉膜由三级栉片构成,位于中央的主栉片和主栉片延伸的次级栉片,以及少数次级栉片延伸出的三级栉片。有1个主栉片,次级栉片18~22个,少数次级栉片可以延伸出2~3个三级栉片。栉膜主要由疏松结缔组织构成,含有丰富的胶原纤维、血管及大量黑色素颗粒。 结论 鸵鸟眼球栉膜的解剖学和组织学结构是研究哺乳动物和人的视网膜血管功能的理想模型。
Objective To investigate the anatomy and histology of 10 ostrichs’ pecten in order to provide morphological base for research of ostrich pecten oculi. Methods The morphology of the pecten oculi was observed with a microscope in the 10-month-old healthy Africa black-neck ostrich (Struthio camelus) by hematoxylin-eosin staining, Gordon-Sweet staining, and Van Gieson staining. Results Gross-anatomically, the pecten oculi was attached to the optic disc, and had an irregular cone shape and projected into the vitreous body. In the steromicroscope investigation, the pecten oculi was composed of 1 primary lamella, 18-22 secondary lamellae and a few tertiary lamellae. The primary lamella was vertically located in the central part of the pecten, the secondary lamellae were peripherally originated from the primary lamella with the tertiary lamellae which arose from the distal part of some secondary lamella. The pecten consisted of loose connective tissue and was rich in collagen fibers, blood vessel and pigment. Conclusion Pecten oculi in the ostrich is an ideal model for the research of the function of retinal blood vessel form mammal and human.
Pecten oculi / Tissue sections / Hematoxylin and eosin staining / African ostrich (Struthio camelus)
[1]Boire D, Dufour JS, Theoret H,et al. Quantitative analysis of the retinal ganglion cell layer in the ostrich, Struthio camelus[J]. Brain Behav Evol, 2001, 58 (6): 343-355.
[2]David B, Meyer.The Visual System in Vertebrates[M]. Berlin: Springer, 1977,7(5):549-611.
[3]Kiama SG, Maina JN, Bhattacharjee J, et al. The morphology of the pecten oculi of the ostrich, Struthio camelus[J]. Ann Anat, 2006, 188(6): 519-528.
[4]Orhan IO, Ekim O, Bayraktarogly AG. Morphological investigation of the pecten oculi in quail (Coturnix coturnix japonica)[J]. Ankara Univ Vet Fak Derg, 2011, 58(1): 5-10.
[5]Mann IC. The function of the pecten[J]. Br J Ophthalmol, 1924, 8(5): 209-226.
[6]Brach V. The effect of intraocular ablation of the pecten oculi of the chicken[J]. Invest Ophthalmol, 1975, 14(2): 166-168.
[7]Tucker R. The surface of the pecten oculi in the pigeon[J]. Cell Tissue Res, 1975, 157(4): 457-465.
[8]Gao Q, Garcia-Pichel F. Microbial ultraviolet sunscreens[J]. Nat Rev Microbiol, 2011, 9(11): 791-802.
[9]Barlow HB, Ostwald TJ. Pecten of the pigeon’s eye as an inter-ocular eye shade[J]. Nat New Biol, 1972, 236(64): 88-90.
[10]Schmitz S, Thomas PD, Allen TM, et al. Dual role of melanins and melanin precursors as photoprotective and phototoxic agents:inhibition of ultraviolet radiation-induced lipid peroxidation[J]. Photochem Photobiol, 1995, 61(6): 650-655.
[11]Krol ES, Liebler DC. Photoprotective actions of natural and synthetic melanins[J]. Chem Res Toxicol, 1998, 11(12): 1434-1440.
[12]Bawa SR, YashRoy RC. Effect of dark and light adaptation on the retina and pecten of chicken[J]. Exp Eye Res, 1972, 13(1): 92-97.
[13]Dayan MO, Ozaydin T. A comparative morphometrical study of the pecten oculi in different avian species[J]. Scientific World Journal, 2013, 2013: 965652.
a-防御素表达紊乱在溃疡性结肠炎小鼠模型发病过程中的作用机制
/
〈 |
|
〉 |