欢迎访问《解剖学报》官方网站！今天是
English
神经纤维丝蛋白、神经烯醇化酶和突触素在人胚胎舌组织中的表达
Expression of neurofilament protein, neuron-specific enolase and synaptophysin in human embryonic tongue tissues
目的 探讨神经纤维丝蛋白（NF）、神经烯醇化酶(NSE)和突触素(SYN)在人胚胎舌组织不同发育阶段的分布特征。 方法 应用免疫组织化学法，检测第2～4个月胎龄段共16份人胚胎舌组织内NF、NSE和SYN蛋白的表达，分析其变化规律。 结果 第2～4个月龄段，NF、NSE和SYN蛋白在人胚舌组织内均有阳性表达。随着胎龄的增大，NF、NSE和SYN在舌组织内阳性表达数量增多，表达强度逐渐增强。第2个月龄时，NF、NSE和SYN蛋白呈少量弱阳性表达，阳性表达强度值分别是135.83±24.62、136.57±15.23和139.84±21.40。第3个月龄时，NF、NSE和SYN阳性表达强度值分别是96.04±23.37、94.89±22.52和90.65±21.08。第4个月胎龄时，NF、NSE和SYN阳性表达强度值分别是79.02±20.90、76.78±21.27和83.43±25.90。应用 One-Way ANOVA和 LSD-t统计学方法，分析第2～4个月龄段人胚胎舌组织内NF、NSE和SYN蛋白的各自阳性表达强度值，P＜0.01。 结论 第2～4个月龄段，人胚胎舌组织内NF、NSE和SYN的表达强度值随胎龄增大而降低，它们均参与调控人胚胎舌内神经系统和舌肌的分化发育。
Objective To explore the distribution of neurofilament protein（NF）, neuron-specific enolase（NSE）and synaptophysin（SYN）proteins at the developmental stages of the human embryonic tongue tissue. Methods Immunohistochemistry was used to detect the expressions of NF, NSE and SYN proteins in the tongue tissues of 16 embryos during the second, third and fourth month of human embryonic and fetal development. Results In the embryos at the 2nd,3nd and 4th months of gestation, NF, NSE and SYN proteins expressed in the tongue tissue. With the increase of gestational age, the number and intensity of the positive expression of NF, NSE and SYN in tongue gradually increased. At the 2nd month of gestation, NF, NSE and SYNproteins were a small amount of weakly positive expression, and the intensity values of their positive expression were 135.83±24.62，136.57±15.23 and 139.84±21.40, respectively. At the 3rd month of gestation, the intensity values of NF, NSE and SYN positive expression were 96.04±23.37，94.89±22.52 and 90.65±21.08, respectively. At the 4th month of gestation, the intensity values of NF, NSE and SYN positive expression were 79.02±20.90，76.78±21.27 and 83.43±25.90, respectively. One-Way ANOVAand LSD-t test were employed to compare the intensity values of NF, NSE and SYN expressions in tongue tissues detected in the 3 fetal periods. The results of NF, NSE and SYN showed significant difference in the tongue tissue(P<0.01). Conclusion At the second to fourth gestation, with the increase of gestational age, the intensity values of NF, NSE and SYN positive expressions are lower. NF, NSE and SYN proteins participate in regulation of the development and differentiation in the nervous system and muscle tissues of tongues in human embryos and fetus.
浙江省教育厅科研计划项目
/
| 〈 |
|
〉 |
