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小鼠肠系膜边缘血管弓通过供血代偿减轻肠缺血再灌注损伤
Mesenteric marginal vessel arch attenuates intestinal ischemia-reperfusion injury by compensating blood-supply
目的 观察肠系膜边缘血管弓通过对肠管供血代偿作用减轻小鼠肠缺血再灌注(I/R)损伤。方法 90只昆明小鼠雌雄各半，随机分为正常A组、假手术B组、模型(C、D、E)组，C组夹闭肠系膜上动脉，D组夹闭回盲瓣旁的两相邻回肠直动脉，E组在D组基础上夹闭两直动脉对应边缘血管弓的近幽门端。由于各个组夹闭动脉不同，动脉营养区的肠管发生缺血再灌注损伤的范围和程度亦不同，由此来观察和比较各组间小鼠肠推进率及其平滑肌慢波、收缩活力以及小肠组织形态学变化。
结果 模型组小鼠肠推进率显著低于A组(P<0.01)，C组肠推进率显著低于D组(P<0.01)，D组肠推进率明显高于E组(P<0.01)。模型组小鼠回肠平滑肌慢波电活动的频率和振幅值显著低于A组(P<0.01)，C组小肠平滑肌慢波电活动的频率和振幅低于D组(P<0.05)，D组则高于E组(P<0.05)。模型组小鼠回肠收缩力的频率和振幅值显著低于A组(P<0.01)，C组小肠收缩力的频率和振幅值均低于D组(P<0.05)，活力值显著低于D组(P<0.01)，D组频率显著高于E组(P<0.01)，活力值显著高于E组(P<0.01)。模型组小肠组织损伤严重，其中C组和E组损伤均较D组严重，差异具有显著统计学意义(P<0.01)。 结论 肠系膜边缘血管弓通过供血代偿作用能够减轻肠缺血再灌注损伤。
Objective To observe the effect of mesenteric marginal vessel arch attenuating intestinal ischemia-reperfusion（I/R）injury by compensating blood-supply for the intestinal canal in mice. MethodsNinety KM rats were randomly divided into normal group(A), sham-operation group(B), and clipping mesenteric artery group(C), clipping the two adjacent straight artery beside ileocecal valve group(D), and clipping the marginal vessel arch correspondence two adjacent straight artery near the pyloric end group(E) based on D group. The range and degree of ischemia-reperfusion in intestinal canal nourished by arteries were different because each group clipped different arteries. According to the different models in each group, the ileum propulsion rate, the frequency and amplitude of the slow wave and contractility were observed. HE staining was used to evaluate the intestinal mucosal injury by histological examination. Results The ileum propulsion rates of the model group(C,D,E group) were lower than that of the A group(P<0.01). Compared with the C group, the ileum propulsion rate of the D group was higher(P<0.05), and compared with E group, D group was higher(P<0.05). The frequency and amplitude of the slow wave of the model group were lower than that of the A group(P<0.01), while compared with the C and E groups, D group was higher(P<0.05). The frequency, amplitude and vitality of the contractility of the model group were lower than that of the A group (P<0.01), the results of D group in comparison with the C and E groups were similar. Compared to A group, the intestinal mucosal injury of the model group was more serious(P<0.01), and both the scores of C and E group were higher than D group(P<0.05). Conclusion Mesenteric marginal vessel arch can attenuate intestinal ischemia-reperfusion injury by compensating blood-supply for the intestinal canal.
四川省教育厅教育质量工程项目---省级大学生创新实验项目
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