当归(粗)多糖对麻黄素肝组织损伤的保护作用

doi:10.16098/j.issn.0529-1356.2015.03.016

解剖学报 ›› 2015 ›› Issue (3) : 379-386. DOI: 10.16098/j.issn.0529-1356.2015.03.016

组织学胚胎学发育生物学

当归(粗)多糖对麻黄素肝组织损伤的保护作用

孟茹¹李重阳² 俞诗源^1,3* 袁静¹ 魏娉婷¹

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Protection of Angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine

MENG Ru¹ LI Chong-yang² YU Shi-yuan^1,3* YUAN Jing¹ WEI Ping-ting¹

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摘要

目的探讨当归多糖对麻黄素致肝损伤的保护作用。方法 72只小鼠分为正常对照组、实验对照组、当归多糖1组和当归多糖2组4个组，实验对照组和当归多糖组连续腹腔注射0.2ml浓度为4.0g/L麻黄素溶液15d(每天2次)，正常对照组注射等量生理盐水，当归多糖1组和2组在腹腔注射麻黄素溶液1h后，灌胃0.3ml(12.5g/L、25.0g/L)当归（粗）多糖溶液，正常对照组和实验对照组灌胃等量的生理盐水。分别于5、10、15d用光学显微镜技术观察各组小鼠肝组织结构的变化，用比色法检测小鼠血浆γ-谷氨酰转移酶(γ-GT)、碱性磷酸酶(AKP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)及肝组织超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的变化，用免疫组织化学方法检测小鼠肝组织Bax蛋白、Csapase-3蛋白表达的变化。结果实验对照组小鼠肝组织有不同程度的损伤，肝细胞索排列紊乱，肝细胞疏松，肝血窦扩张，血浆γ-GT、AKP、ALT、AST活性明显升高，肝组织SOD活性显著降低，MDA含量显著升高，Bax、Caspase-3蛋白的表达显著升高；当归多糖组小鼠肝组织病理损伤明显减轻，血浆γ-GT、AKP、ALT、AST活性明显降低，肝组织SOD活性明显升高，MDA含量显著降低，Bax、Caspase-3蛋白的表达有所降低。结论当归（粗）多糖可提高肝组织细胞的抗氧化能力，抑制细胞凋亡，促进组织细胞损伤修复，对麻黄素致肝损伤具有一定的保护作用。

Abstract

Objective To investigate the protection of Angelica sinensis polysaccharide(ASP) on ephedrine induced hepatic injury in mice. Methods Seventy-two mice were randomly divided into 4 groups：the natural control group，experiment control group，ASP1 group， and ASP2 group.The experimental control group and ASP groups were intraperitoneally injected with a 4.0g/L of ephedrine 0.2ml twice a day for 15 days， and the natural control group was injected with a same amount of saline.The ASP groups were irrigated to stomach with 0.3ml Angelica sinensispolysaccharide solution (12.5g/L，25.0g/L)1 hour after ephedrine injection，the natural control group and experiment control group were irrigated to stomach with a same amount of saline.The mice were sacrificed at 5 day，10day，and 15 day after treatment，respectively.The morphological observation was used to observe pathological changes of hepatic tissues.Activities of hepatic histologicalsuperoxide dismutase（SOD）and plasma γ-glutamyltransferase(γ-GT), alkaline phosphatase (AKP), glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminase(AST)，as well as content of malonaldehyde（MDA）were detected by colorimetry.The expressions of Bax protein and Caspase-3 protein were measured by immunohistochemistry in liver of mice. Results There were various degree damages in hepatic tissues，hepatic cords were disorganized，liver cells were loose and liver sinusoid expanded，the activity of γ-GT、AKP、ALT、AST in plasma were significantly increased，the activity of SOD in liver tissue was significantly reduced，the content of MDA in liver tissue was increased significantly，the expression of Bax and Caspase-3 protein in liver were significantly increased.Compared with the experimental control group，pathological damage of liver was significantly improved，the activity of plasma γ-GT, AKP, ALT, AST were significantly reduced，the activity of SOD in liver tissue was increased significantly，however the content of MDA was significantly decreased and the expressions of Bax and Caspase-3 protein in liver were reduced in Angelica sinensis polysaccharide group. ConclusionAngelica sinensis polysaccharide can improve liver tissue antioxidant capacity and promote reparation of cell injury and protect hepatic injury which is induced by ephedrine.

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孟茹李重阳俞诗源* 袁静魏娉婷. 当归(粗)多糖对麻黄素肝组织损伤的保护作用[J]. 解剖学报. 2015(3): 379-386 https://doi.org/10.16098/j.issn.0529-1356.2015.03.016

MENG Ru LI Chong-yang YU Shi-yuan* YUAN Jing WEI Ping-ting. Protection of Angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine[J]. Acta Anatomica Sinica. 2015(3): 379-386 https://doi.org/10.16098/j.issn.0529-1356.2015.03.016

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