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中药复方液对麻黄素仔鼠肝组织结构和抗氧化酶活性的影响
Effects of a Chinese medicine compound on histological structure and antioxidant enzyme activity in the liver of filial mice injured by ephedrine
目的 探讨中药复方液对麻黄素致损伤的影响。 方法 采用递增剂量连续腹腔注射浓度为2.0g/L、3.0g/L和4.0g/L的麻黄素溶液，在注射麻黄素1h 后再分别灌胃，浓度为20.0g/L、30.0g/L和40.0g/L的中药复方液。分别于灌胃中药5、10、15d后，用比色法测定仔鼠肝组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量，用显微镜观察肝组织结构，用免疫组织化学法检测肝组织c-Fos蛋白的表达。 结果 麻黄素组仔鼠10d、15d 时肝组织SOD、CAT的活性低于对照组，MDA含量高于对照组 (P<0.05或 P<0.01)，肝组织有不同程度的损伤，肝组织c-Fos蛋白表达强度高于对照组 (P<0.05或P<0.01)；中药组仔鼠肝组织SOD、CAT活性升高，MDA含量降低(P<0.05或P<0.01)，肝组织损伤明显减轻，肝组织c-Fos蛋白的阳性表达强度减弱(P<0.05或P<0.01)。结论 麻黄素对肝组织有明显的损伤效应，中药复方液可提高细胞抗氧化活性，具有抗炎、抗氧化和保护肝细胞等作用，能有效减轻麻黄素对肝组织的损伤。
Objective To investigate the effect of a Chinese medicine compound on histological damnification of ephedrine, and understand an important significance for forfend freak-out and safeguard social stability. Methods The filial mice were intraperitoneal injected escalation doses (2.0 g/L，3.0 g/L，4.0 g/L ) of ephedrine，and irrigated to stomach with escalation doses（20.0 g/L，30.0 g/L and 40.0 g/L）of Chinese medicine compounds after injected ephedrine 1 hour later.The samples were withdrawn at 5，10 and 15 days respectively，the activities of superoxide dismutase(SOD)，catalase(CAT)，as well as content of malondialdehyde(MDA) were determined by colorimetry and the liver structures of filial mice were observed by optical microscopy，the expression changes of c-Fos protein were measured by immunohistochemical staining. Results The activity of SOD，CAT of ephedrine groups was lower than that of the control groups in 10 days，15 days，while the content of MDA was higher than control groups (P<0.05 or P<0.01). The filial mice’s liver histology appeared various degrees of damage, and the expression intensity of c-Fos protein in liver of the ephedrine groups was higher than that of the the control groups (P<0.05 or P<0.01).The activity of SOD，CAT of the Chinese medicine groups increased，while the content of MDA decreased (P<0.05 or P<0.01).The liver damage of filial mice of Chinese medicine groups reduced，the expression of c-Fos protein intensity decreased (P<0.05 or P<0.01). Conclusion Ephedrine affects the histological structure and antioxidant enzymes activity of the liver tissue.The Chinese medicine compound may enhance cells antioxidant enzymes activity, and reduce liver tissue damage by injecting ephedrine.
中药复方 / 麻黄素 / 肝脏 / 酶活性 / c-Fos蛋白 / 分光光度法 / 仔鼠
Chinese medicine compound / Ephedrine / Liver / Enzyme activity / c-Fos protein / Spectrophotometer / Filial mouse
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