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不同年龄牦牛睾丸蛋白基因产物9.5和神经肽Y的分布比较
Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages
目的 探讨蛋白基因产物9.5(PGP9.5)和神经肽Y(NP-Y)在不同年龄牦牛睾丸的分布特征。方法 采取睾丸摘除术收集3~4月龄睾丸9对，3、4岁牦牛睾丸10对，10~12岁牦牛睾丸7对，常规石蜡包埋、切片，免疫组织化学SP法观察PGP 9.5和NP-Y在不同年龄牦牛睾丸的分布特点。结果 PGP 9.5和NP-Y显著分布于幼龄及青年牦牛睾丸Leydig细胞及间质血管周围或血管壁内，两者在青年牦牛睾丸初级精母细胞为阴性，其余生精细胞为阳性；且阳性信号在睾丸体最强，其次为头部，尾部最弱；各年龄段Sertoli 细胞中NP-Y为阳性表达，PGP 9.5均为阴性；老龄牦牛睾丸血管PGP 9.5阳性信号明显，而NP-Y基本无表达。结论 青年牦牛睾丸组织PGP 9.5及NP-Y的显著分布对睾丸生殖功能调节发挥着重要的调控作用；提示NP-Y在老龄牦牛睾丸血管的分布显著降低。
Objective To investigate the distribution of gene product 9.5 and neuropeptide Y(NP-Y) in yak testis and in an attempt of exploring their relationships with testicular spermatogenesis at different ages. Methods Using immunohistochemical SP technique, we depicted the distribution characteristics of PGP 9.5 and NP-Y in yak testis: 9 pairs of yak calves in age from 3 to 4 months, 10 pairs of young adult yak from 3 to 4 years and 7 pairs of aging yak from 9 to 13 years old were prepared by orchiectomy surgery, bedded in paraffin and sectioned serially. Results NP-Y and PGP 9.5 immunoreactivities were abundantly distributed in gonads, mainly associated with Leydig cell nests, blood vessels, and seminiferous tubules. PGP 9.5 and NP-Y were mainly expressed in spermatogonia of yak calves testis and in spermatogenic cells of young yak testis. By contrast, no NP-Y and PGP 9.5 immunoreactivities were observed within the primary spermatocytes. The expression of PGP 9.5 and NP-Y in corpus testis were stronger than the caput and cauda of the testis. A small number of PGP 9.5-immunoreactive cells was detected in testis vscellum of aging yak. Conclusion Our results suggest that the PGP 9.5 and NP-Y expression is significantly different in young adult yak testis and may serve as regulators for testicular functions.The significant dcrease of distribution of NP-Y in the wall of vascular structure in aging yak testis.
蛋白基因产物9.5 / 神经肽Y / 睾丸 / 年龄 / 免疫组织化学 / 牦牛
Protein gene product 9.5 / / Neuropeptide Y / / Testis / / Age / / Immunohistochemistry / Yak
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