目的 探索用于膜片钳记录的脑片制备方法并分析鼠龄、溶液、操作过程等因素对神经细胞活性的影响。 方法 取各年龄段的Sprague-Dawley大鼠60只，麻醉后快速断头取脑，冰镇后取出并修块，用振动切片机切取300μm下丘脑冠状脑片，人工脑脊液33℃孵育45min后，在红外微分干涉相差显微镜下观察脑片神经元。
结果 镜下可观察到状态良好的神经元（边界清晰、立体感强、细胞核和颗粒不可见），神经元活性的好坏与实验条件和实验操作有关。 结论 鼠龄、溶液、实验操作等因素与脑片神经元活性密切相关，实验过程中需要严格控制各种实验条件。

  Objective To explore a method of preparing brain slices for patch clamp technique and to analyze effects of age,solutions and processes of operation on neurons of brain slices. Methods Sixty Sprague-Dawley rats were anesthetized and quickly decapitated. Their brains were cooled and dissected then 300μm coronary brain slices were cut and incubated in the artifical cerebrospinal fluid at 33℃ for 45 minutes. Neurons were observed under an infrared differential aberration interference microscope. Results Neurons showed clear boundary, and three-dimensional appearance and nuclei and particles were not seen. The activity of brain slices depended on various experiment conditions.Conclusion The activity of neurons in the brain slice is associated with age,solutions and processes of operation, thus in experiments we should strictly control these factors.